Ningnanmycin is a novel biochemical pesticide which was now used extensively in China. A fast and simple method using high-performance liquid chromatography with ultraviolet detection coupled with solid phase extraction was developed and validated for determination of ningnanmycin in cucumber and soil. The recoveries of ningnanmycin from the fortified cucumber and soil samples ranged from 80.7 % to 107.7 % with relative standard deviations less than 6.6 %. Limits of quantification of the method for both cucumber and soil were 0.02 mg kg. The proposed method was successfully applied to determine the dissipation and residues of ningnanmycin in cucumber and soil under field conditions. Direct confirmation of the analytes in samples was realized by liquid chromatography-mass spectrometry. [ABSTRACT FROM AUTHOR]
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