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Tytuł pozycji:

Lipoxygenase H1 gene silencing reveals a specific role in supplying fatty acid hydroperoxides for aliphatic aldehyde production.

Tytuł :
Lipoxygenase H1 gene silencing reveals a specific role in supplying fatty acid hydroperoxides for aliphatic aldehyde production.
Autorzy :
León J; Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, Universidad Autónoma de Madrid Colmenar Viejo km 15,500, 28049 Madrid, Spain.
Royo J
Vancanneyt G
Sanz C
Silkowski H
Griffiths G
Sánchez-Serrano JJ
Pokaż więcej
Źródło :
The Journal of biological chemistry [J Biol Chem] 2002 Jan 04; Vol. 277 (1), pp. 416-23. Date of Electronic Publication: 2001 Oct 23.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology
Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology
MeSH Terms :
Aldehydes/*metabolism
Lipid Peroxides/*metabolism
Lipoxygenase/*physiology
Solanum tuberosum/*enzymology
Immunohistochemistry ; Lipoxygenase/analysis ; Lipoxygenase/genetics ; Phenotype ; Plants, Genetically Modified ; RNA Splicing ; Substrate Specificity
Substance Nomenclature :
0 (Aldehydes)
0 (Lipid Peroxides)
EC 1.13.11.- (13-lipoxygenase)
EC 1.13.11.12 (Lipoxygenase)
Entry Date(s) :
Date Created: 20011025 Date Completed: 20020124 Latest Revision: 20210209
Update Code :
20210211
DOI :
10.1074/jbc.M107763200
PMID :
11675388
Czasopismo naukowe
Lipoxygenases catalyze the formation of fatty acid hydroperoxide precursors of an array of compounds involved in the regulation of plant development and responses to stress. To elucidate the function of the potato 13-lipoxygenase H1 (LOX H1), we have generated transgenic potato plants with reduced expression of the LOX H1 gene as a consequence of co-suppression-mediated gene silencing. Three independent LOX H1-silenced transgenic lines were obtained, having less than 1% of the LOX H1 protein present in wild-type plants. This depletion of LOX H1 has no effect on the basal or wound-induced levels of jasmonates derived from 13-hydroperoxylinolenic acid. However, LOX H1 depletion results in a marked reduction in the production of volatile aliphatic C6 aldehydes. These compounds are involved in plant defense responses, acting as either signaling molecules for wound-induced gene expression or as antimicrobial substances. LOX H1 protein was localized to the chloroplast and the protein, expressed in Escherichia coli, showed activity toward unesterified linoleic and linolenic acids and plastidic phosphatidylglycerol. The results demonstrate that LOX H1 is a specific isoform involved in the generation of volatile defense and signaling compounds through the HPL branch of the octadecanoid pathway.

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