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Tytuł pozycji:

Immunohistochemical detection of the retinoid acid receptors (RXR-alpha, -beta, -gamma) and Farnesoid X-activated receptor (FXR) in the marbled newt along the annual cycle.

Tytuł:
Immunohistochemical detection of the retinoid acid receptors (RXR-alpha, -beta, -gamma) and Farnesoid X-activated receptor (FXR) in the marbled newt along the annual cycle.
Autorzy:
Alfaro JM; Department of Cell Biology and Genetics, University of Alcalá, Madrid, Spain.
Ricote M
Lobo MV
Royuela M
Fraile B
Paniagua R
Arenas MI
Źródło:
Molecular reproduction and development [Mol Reprod Dev] 2002 Jun; Vol. 62 (2), pp. 216-22.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: <2005-> : Hoboken, N.J. : Wiley-Liss
Original Publication: New York, NY : A.R. Liss, 1988-
MeSH Terms:
DNA-Binding Proteins/*analysis
Receptors, Cytoplasmic and Nuclear/*analysis
Receptors, Retinoic Acid/*analysis
Testis/*chemistry
Transcription Factors/*analysis
Animals ; Blotting, Western/methods ; Immunoenzyme Techniques ; Male ; Retinoid X Receptors ; Salamandridae
Substance Nomenclature:
0 (DNA-Binding Proteins)
0 (Receptors, Cytoplasmic and Nuclear)
0 (Receptors, Retinoic Acid)
0 (Retinoid X Receptors)
0 (Transcription Factors)
0C5V0MRU6P (farnesoid X-activated receptor)
Entry Date(s):
Date Created: 20020502 Date Completed: 20021104 Latest Revision: 20181130
Update Code:
20240104
DOI:
10.1002/mrd.10104
PMID:
11984832
Czasopismo naukowe
Retinoid acid receptors (RXR-alpha, -beta, -gamma) and Farnesoid X-activated receptor (FXR) expression in the testis of the marbled newt were investigated with special attention to the changes during the annual testicular cycle, using light microscopy immunohistochemistry and Western blot analysis. The annual testicular cycle of the marbled newt (Triturus marmoratus marmoratus) comprises three periods: (a) proliferative period (germ cell proliferation from primordial germ cells to round spermatids, April-June); (b) spermiogenesis period (July-September); and (c) quiescence period (interstitial and follicular cells form the glandular tissue, October-April). In the proliferative period, primordial germ cells and primary spermatogonia immunostained intensely to the three types of RXRs and also to FXR. In the other periods, immunostaining to these antibodies was weak or absent. Secondary spermatogonia stained weakly to the four antibodies in the proliferative period, and only to FXR, also weakly, in the spermiogenesis period. Immunoreactive primary spermatocytes were weakly labeled with the RXR antibodies in the proliferative period. Spermatids and spermatozoa did not stain to any antibody in any period. Follicular cells only immunostained to RXR-gamma and only in the quiescence period when they are forming the glandular tissue, together with the interstitial cells. As follicular cells, interstitial cells only immunostained in the quiescence period; however, they immunoreacted to the three types of RXRs. These findings suggest that in the newt, RXRs and FXR are involved in spermatogenesis control by regulating the proliferation of primordial germ cells and spermatogonia. In addition, RXR-gamma seems to be also involved in the development of the glandular (steroidogenic) tissue.
(Copyright 2002 Wiley-Liss, Inc.)

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