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Tytuł pozycji:

Proinflammatory cytokines in the course of Mycobacterium tuberculosis-induced apoptosis in monocytes/macrophages.

Tytuł:
Proinflammatory cytokines in the course of Mycobacterium tuberculosis-induced apoptosis in monocytes/macrophages.
Autorzy:
Ciaramella A; Department of Biology, University of Rome Tor Vergata, Rome, Italy.
Cavone A
Santucci MB
Amicosante M
Martino A
Auricchio G
Pucillo LP
Colizzi V
Fraziano M
Źródło:
The Journal of infectious diseases [J Infect Dis] 2002 Nov 01; Vol. 186 (9), pp. 1277-82. Date of Electronic Publication: 2002 Oct 03.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: Jan. 2011- : Oxford : Oxford University Press
Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press
MeSH Terms:
Apoptosis/*physiology
Cytokines/*biosynthesis
Macrophages/*microbiology
Monocytes/*microbiology
Mycobacterium tuberculosis/*immunology
Apoptosis/drug effects ; Cell Survival/drug effects ; Cells, Cultured ; Cysteine Proteinase Inhibitors/pharmacology ; Enzyme Inhibitors/pharmacology ; Humans ; In Vitro Techniques ; Macrophages/cytology ; Macrophages/immunology ; Monocytes/cytology ; Monocytes/immunology
Substance Nomenclature:
0 (Cysteine Proteinase Inhibitors)
0 (Cytokines)
0 (Enzyme Inhibitors)
Entry Date(s):
Date Created: 20021029 Date Completed: 20021209 Latest Revision: 20141120
Update Code:
20240104
DOI:
10.1086/344645
PMID:
12402197
Czasopismo naukowe
Mycobacterium tuberculosis (MTB) can induce apoptosis in monocytes/macrophages both in vitro and in vivo, and this phenomenon is associated with mycobacterial survival. The present study addresses the possibility that apoptotic and inflammatory pathways could coexist through a caspase-1-mediated mechanism. In this context, a caspase-1 inhibitor (YVAD), but not caspase-3 (DEVD) or caspase-4 (LEVD) inhibitors, was able to strongly inhibit MTB-induced apoptosis. Moreover, caspase-1 activity was confirmed by the increased maturation of interleukin (IL)-1beta. Of interest, IL-1beta and tumor necrosis factor (TNF)-alpha were produced massively in the course of infection, and both were inhibited by YVAD pretreatment. To determine whether TNF-alpha was produced actively by apoptotic cells, the intracytoplasmatic cytokine content and apoptotic phenotype were analyzed at the single-cell level. Results showed a progressive increase of TNF-alpha production in annexin V-positive cells. These results indicate that MTB-induced apoptosis is associated with proinflammatory cytokine production.

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