Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions.

Szczegóły
Abstrakt

Tytuł:: Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions.
Autorzy:: Fedorova O; Department of Biochemistry and Molecular Biophysics, 630 West 168 Street, Box 36, Columbia University, New York, NY 10032, USA.
Su LJ
Pyle AM
Źródło:: Methods (San Diego, Calif.) [Methods] 2002 Nov; Vol. 28 (3), pp. 323-35.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review
Język:: English
Imprint Name(s):: Publication: Duluth, MN : Academic Press
Original Publication: San Diego : Academic Press, c1990-
MeSH Terms:: Introns*
RNA, Catalytic/*chemistry
Catalysis ; Hydrolysis ; Kinetics ; Nucleic Acid Conformation ; RNA Splicing ; RNA, Catalytic/physiology ; Structure-Activity Relationship ; Substrate Specificity
Liczba referencji:: 78
Grant Information:: T32 GM08281 United States GM NIGMS NIH HHS
Substance Nomenclature:: 0 (RNA, Catalytic)
Entry Date(s):: Date Created: 20021115 Date Completed: 20030508 Latest Revision: 20191106
Update Code:: 20240104
DOI:: 10.1016/s1046-2023(02)00239-6
PMID:: 12431436
: Czasopismo naukowe

Full Text Finder

Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group II introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5gamma group II intron.

Informacja