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Tytuł:
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Real-time multicolor DNA detection with chemoresponsive diffraction gratings and nanoparticle probes.
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Autorzy:
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Bailey RC; Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208, USA.
Nam JM
Mirkin CA
Hupp JT
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Źródło:
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Journal of the American Chemical Society [J Am Chem Soc] 2003 Nov 05; Vol. 125 (44), pp. 13541-7.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
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Język:
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English
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Imprint Name(s):
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Publication: Washington, DC : American Chemical Society
Original Publication: Easton, Pa. [etc.]
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MeSH Terms:
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Antigens, Bacterial*
DNA/*analysis
DNA, Single-Stranded/*chemistry
Nucleic Acid Hybridization/*methods
Bacterial Toxins/genetics ; DNA/chemistry ; DNA Probes/chemistry ; Lasers ; Nanotechnology/methods ; Particle Size ; Spectrophotometry, Ultraviolet
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Substance Nomenclature:
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0 (Antigens, Bacterial)
0 (Bacterial Toxins)
0 (DNA Probes)
0 (DNA, Single-Stranded)
0 (anthrax toxin)
9007-49-2 (DNA)
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Entry Date(s):
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Date Created: 20031030 Date Completed: 20040226 Latest Revision: 20061115
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Update Code:
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20240104
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DOI:
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10.1021/ja035479k
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PMID:
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14583051
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We report a real-time DNA detection method that utilizes single-strand DNA-modified nanoparticle probes and micropatterned chemoresponsive diffraction gratings interrogated simultaneously at multiple laser wavelengths. The surface-bound nanoparticle probe based assay with the chemoresponsive diffraction grating signal transduction scheme results in an experimentally simple DNA detection protocol, displaying attributes of both detection methodologies: the high sensitivity and selectivity afforded by nanoparticle probes and the experimental simplicity, wavelength-dependent resonant enhancement features, and miniaturization potential provided by the diffraction-based sensing technology.