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Title of the item:

Different resistance mutations can be detected simultaneously in the blood and the lung of HIV-1 infected individuals on antiretroviral therapy.

Title :
Different resistance mutations can be detected simultaneously in the blood and the lung of HIV-1 infected individuals on antiretroviral therapy.
Authors :
White NC; Jefferiss Trust Laboratories, Wright-Fleming Institute, Faculty of Medicine, Imperial College School of Medicine, St. Mary's Hospital, London, United Kingdom.
Israel-Biet D
Coker RJ
Mitchell DM
Weber JN
Clarke JR
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Source :
Journal of medical virology [J Med Virol] 2004 Mar; Vol. 72 (3), pp. 352-7.
Publication Type :
Journal Article
Language :
English
Imprint Name(s) :
Publication: New York Ny : Wiley-Liss
Original Publication: New York, Liss.
MeSH Terms :
Anti-HIV Agents/*therapeutic use
Drug Resistance, Viral/*genetics
HIV Infections/*drug therapy
HIV Infections/*virology
HIV-1/*genetics
Leukocytes, Mononuclear/*virology
Lung/*virology
Anti-HIV Agents/administration & dosage ; Antiretroviral Therapy, Highly Active ; Bronchoalveolar Lavage Fluid/virology ; Genotype ; HIV Reverse Transcriptase/genetics ; HIV-1/drug effects ; HIV-1/growth & development ; HIV-1/isolation & purification ; Humans ; Mutation ; RNA, Viral/chemistry ; RNA, Viral/isolation & purification ; Retrospective Studies ; Reverse Transcriptase Inhibitors/therapeutic use ; Viremia
Substance Nomenclature :
0 (Anti-HIV Agents)
0 (RNA, Viral)
0 (Reverse Transcriptase Inhibitors)
EC 2.7.7.49 (HIV Reverse Transcriptase)
Entry Date(s) :
Date Created: 20040130 Date Completed: 20040329 Latest Revision: 20071115
Update Code :
20210210
DOI :
10.1002/jmv.20010
PMID :
14748057
Academic Journal
In this retrospective study, matched peripheral blood and lung samples from patients on antiretroviral therapy were studied in order to investigate whether differences in mutations associated with resistance to nucleoside analogues could be detected between the lung and blood. Discordant mutation patterns in the reverse transcriptase (RT) between plasma and cell free bronchoalveolar lavage fluid (BAL-fluid) HIV-1 genomic RNA was observed in five out of seven patients on nucleoside reverse transcriptase inhibitor (NRTI) monotherapy and six out of seven on combination therapy. In the cellular compartments, DNA recovered from peripheral blood mononuclear cells (PBMCs) and cells from BAL-cells discordant HIV-1 resistance genotypes were detected in 15 out of 44 matched samples. Differences in resistant genotypes between PBMCs and BAL-cells were most pronounced in patients receiving combination antiretroviral therapy. The pattern and number of mutations in RT associated with resistance differed in the BAL-cells compared to PBMCs in four out of 12 subjects not receiving antiretroviral therapy at the time of bronchoscopy, three from 14 patients on NRTI monotherapy, five out of nine on dual combination therapy and three out of nine on HAART. The differences in the detection of resistance mutations between blood and the lung suggest that the lung is a site of replication for HIV-1.
(Copyright 2004 Wiley-Liss, Inc.)

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