Effects of STI571 and p27 gene clone on proliferation and apoptosis of K562 cells.

Szczegóły
Abstrakt

Tytuł:: Effects of STI571 and p27 gene clone on proliferation and apoptosis of K562 cells.
Autorzy:: Wang W; Department of Hematology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
Yao LB
Liu XP
Feng Q
Shang ZC
Cao YX
Sun BZ
Źródło:: World journal of gastroenterology [World J Gastroenterol] 2005 Apr 14; Vol. 11 (14), pp. 2130-5.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: 2014- : Pleasanton, CA : Baishideng Publishing Group
Original Publication: Beijing : WJG Press, c1998-
MeSH Terms:: Antineoplastic Agents/*pharmacology
Cell Cycle Proteins/*genetics
Genetic Therapy/*methods
Leukemia, Erythroblastic, Acute/*drug therapy
Piperazines/*pharmacology
Pyrimidines/*pharmacology
Tumor Suppressor Proteins/*genetics
Apoptosis/drug effects ; Base Sequence ; Benzamides ; Cell Division/drug effects ; Cyclin-Dependent Kinase Inhibitor p27 ; Humans ; Imatinib Mesylate ; In Vitro Techniques ; K562 Cells ; Molecular Sequence Data ; Transfection
References:: Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2797-802. (PMID: 10688900)
Mol Ther. 2000 Aug;2(2):161-9. (PMID: 10947944)
Med Sci (Paris). 2003 Feb;19(2):173-86. (PMID: 12836612)
Mol Cancer. 2003 Sep 17;2:32. (PMID: 14521721)
Cell Death Differ. 1997 Oct;4(7):548-54. (PMID: 14555967)
Oncogene. 1999 Mar 4;18(9):1663-76. (PMID: 10208428)
Blood. 1998 Mar 1;91(5):1700-5. (PMID: 9473236)
J Invest Dermatol. 2004 Feb;122(2):400-5. (PMID: 15009722)
J Neuropathol Exp Neurol. 1999 Jul;58(7):691-6. (PMID: 10411338)
Science. 1990 Mar 2;247(4946):1079-82. (PMID: 2408149)
Nature. 1996 Jul 25;382(6589):325-31. (PMID: 8684460)
Ann Hematol. 2000 Jul;79(7):345-54. (PMID: 10965782)
Blood. 2000 Mar 1;95(5):1758-66. (PMID: 10688835)
Blood. 2001 Sep 1;98(5):1524-31. (PMID: 11520803)
Leuk Lymphoma. 2004 Apr;45(4):655-60. (PMID: 15160936)
Oncogene. 1999 Aug 26;18(34):4839-47. (PMID: 10490817)
N Engl J Med. 2001 Apr 5;344(14):1038-42. (PMID: 11287973)
Clin Cancer Res. 2002 Dec;8(12 ):3841-9. (PMID: 12473598)
Blood. 2000 Sep 1;96(5):1933-9. (PMID: 10961897)
Blood. 1997 Dec 15;90(12):4947-52. (PMID: 9389713)
Cancer Res. 1996 Jan 1;56(1):100-4. (PMID: 8548747)
Cell. 1994 Jul 15;78(1):59-66. (PMID: 8033212)
Nature. 1998 Nov 12;396(6707):177-80. (PMID: 9823898)
Head Neck. 2003 Jan;25(1):44-9. (PMID: 12478543)
Blood. 2000 Sep 15;96(6):2246-53. (PMID: 10979973)
Cancer Res. 2000 Apr 1;60(7):2049-55. (PMID: 10766197)
Br J Haematol. 1998 Feb;100(2):295-303. (PMID: 9488616)
Leuk Res. 2004 Jun;28(6):623-9. (PMID: 15120940)
Oncogene. 1997 May 15;14(19):2283-9. (PMID: 9178904)
J Biol Chem. 2001 Jun 29;276(26):23572-80. (PMID: 11323429)
Nat Med. 1996 May;2(5):561-6. (PMID: 8616716)
Substance Nomenclature:: 0 (Antineoplastic Agents)
0 (Benzamides)
0 (Cell Cycle Proteins)
0 (Piperazines)
0 (Pyrimidines)
0 (Tumor Suppressor Proteins)
147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
8A1O1M485B (Imatinib Mesylate)
Entry Date(s):: Date Created: 20050406 Date Completed: 20050518 Latest Revision: 20190430
Update Code:: 20240104
PubMed Central ID:: PMC4305782
DOI:: 10.3748/wjg.v11.i14.2130
PMID:: 15810079
: Czasopismo naukowe

Aim: To investigate the combined effect of STI571 and p27 gene clone on the regulation of proliferation, cell cycle and apoptosis of K562 cell line.
Methods: p27 gene was obtained by RT-PCR, and its sequence was approved to be correct. Then p27-pcDNA3.1 vector was constructed and transfected into K562 cell line. p27-pcDNA3.1-K562 cell clone was screened by G418 after transfection, p27 protein was identified by Western blot. MTT was used to detect the survival rate of the cell. Flow cytometry was used to detect cell cycle and apoptosis index.
Results: The expression of p27 protein could be detected by Western blot in p27-pcDNA3.1-K562 cells. A strong inhibition of cell proliferation was observed in p27-pcDNA3.1-K562 cells as compared with that of the control (pcDNA3.1-K562 cells). The cells at G0/G1 phase were significantly increased, and cells at S phase were greatly declined. The apoptosis index was increased greatly after p27-pcDNA3.1-K562 cells were treated with STI571, and survival rate of the cell was markedly declined (0.35-0.58, P<0.05-0.048 vs STI571-K562 cell, 0.35-0.72, P<0.01-0.001 vs p27-K562 cell).
Conclusion: p27 and STI571 have a synergistic action on inhibition of proliferation and induction of apoptosis on K562 cells.

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