Evaluation of amplified ribosomal DNA restriction analysis as a method for the identification of Botryosphaeria species.

Szczegóły
Abstrakt

Tytuł:: Evaluation of amplified ribosomal DNA restriction analysis as a method for the identification of Botryosphaeria species.
Autorzy:: Alves A; Centro de Biologia Celular, Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. />Phillips AJ
Henriques I
Correia A
Źródło:: FEMS microbiology letters [FEMS Microbiol Lett] 2005 Apr 15; Vol. 245 (2), pp. 221-9.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: 2015- : Oxford Oxford University Press
Original Publication: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies.
MeSH Terms:: Polymerase Chain Reaction*
Polymorphism, Restriction Fragment Length*
Ascomycota/*classification
Ascomycota/*isolation & purification
DNA, Ribosomal/*genetics
Ascomycota/genetics ; Cluster Analysis ; DNA Fingerprinting ; DNA Restriction Enzymes/metabolism ; DNA, Fungal/chemistry ; DNA, Fungal/genetics ; DNA, Ribosomal Spacer/genetics ; Genes, rRNA ; Haplotypes ; Molecular Sequence Data ; Polymorphism, Genetic ; RNA, Ribosomal, 28S/genetics ; Sequence Analysis, DNA
Molecular Sequence:: GENBANK AY928043; AY928044; AY928045; AY928046; AY928047; AY928048; AY928049; AY928050; AY928051; AY928052; AY928053; AY928054
Substance Nomenclature:: 0 (DNA, Fungal)
0 (DNA, Ribosomal)
0 (DNA, Ribosomal Spacer)
0 (RNA, Ribosomal, 28S)
EC 3.1.21.- (DNA Restriction Enzymes)
Entry Date(s):: Date Created: 20050420 Date Completed: 20050922 Latest Revision: 20061115
Update Code:: 20240104
DOI:: 10.1016/j.femsle.2005.03.005
PMID:: 15837376
: Czasopismo naukowe

The polymerase chain reaction was used to amplify a rDNA fragment containing the internal transcribed spacers (ITS1-5.8S-ITS2) and the D1/D2 variable domains of the 28S rDNA from 10 species of the genus Botryosphaeria (Fungi, Ascomycota). Restriction analysis of the amplicons with frequent-cutting endonucleases (amplified ribosomal DNA restriction analysis) allowed the definition of 12 rDNA haplotypes. Each of the rDNA haplotypes could be unambiguously assigned to a single Botryosphaeria species, thus allowing clear identification of all the species tested. Intraspecific polymorphism was very low and detected only in Botryosphaeria parva and Botryosphaeria dothidea. Cluster analysis of banding patterns of the isolates corresponded well with known species delineations. The method described in this paper provides a simple and rapid procedure for the differentiation and identification of Botryosphaeria isolates at the species level.

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