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Tytuł pozycji:

In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus.

Tytuł:
In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus.
Autorzy:
Park YM; Department of Biochemistry, College of Pharmacy, Kyung-Hee University, Dongdaemun-Ku, Hoegi-Dong, Seoul 130-701, South Korea.
Won JH
Kim YH
Choi JW
Park HJ
Lee KT
Źródło:
Journal of ethnopharmacology [J Ethnopharmacol] 2005 Oct 03; Vol. 101 (1-3), pp. 120-8.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.
MeSH Terms:
Analgesics/*pharmacology
Anti-Inflammatory Agents/*pharmacology
Polyporales/*chemistry
Active Transport, Cell Nucleus/drug effects ; Animals ; Cell Line ; Cyclooxygenase 2/analysis ; Cyclooxygenase 2/genetics ; Dinoprostone/biosynthesis ; Male ; Mice ; Mice, Inbred ICR ; NF-kappa B/metabolism ; Nitric Oxide/biosynthesis ; Nitric Oxide Synthase Type II/analysis ; Nitric Oxide Synthase Type II/genetics ; Rats ; Rats, Sprague-Dawley ; Transcription Factor RelA/metabolism ; Tumor Necrosis Factor-alpha/biosynthesis
Substance Nomenclature:
0 (Analgesics)
0 (Anti-Inflammatory Agents)
0 (NF-kappa B)
0 (Transcription Factor RelA)
0 (Tumor Necrosis Factor-alpha)
31C4KY9ESH (Nitric Oxide)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
EC 1.14.99.1 (Cyclooxygenase 2)
K7Q1JQR04M (Dinoprostone)
Entry Date(s):
Date Created: 20050521 Date Completed: 20051228 Latest Revision: 20131121
Update Code:
20240104
DOI:
10.1016/j.jep.2005.04.003
PMID:
15905055
Czasopismo naukowe
The mushroom Inonotus obliquus (Fr.) Pilát (Hymenochaetaceae), has been traditionally used for the treatment of gastrointestinal cancer, cardiovascular disease and diabetes in Russia, Poland and most of Baltic countries. This study was designed to investigate the anti-inflammatory and anti-nociceptive effects of the methanol extract from Inonotus obliquus (MEIO) in vivo and in vitro. MEIO (100 or 200 mg/(kgday), p.o.) reduced acute paw edema induced by carrageenin in rats, and showed analgesic activity, as determined by an acetic acid-induced abdominal constriction test and a hot plate test in mice. To reveal the mechanism of the anti-inflammatory effect of MEIO, we examined its effect on lipopolysaccharide (LPS)-induced responses in a murine macrophage cell line RAW 264.7. MEIO was found to significantly inhibit the productions of nitric oxide (NO), prostaglandin E2 (PGE2) and tumor necrosis factor-alpha (TNF-alpha) in LPS-stimulated RAW 264.7 macrophages. Consistent with these observations, MEIO potently inhibited the protein and mRNA expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, MEIO inhibited the LPS-induced DNA binding activity of nuclear factor-kappaB (NF-kappaB), and this was associated with the prevention of inhibitor kappaB degradation and a reduction in nuclear p65 protein levels. Taken together, our data indicate that the anti-inflammatory and anti-nociceptive properties of MEIO may be due to the inhibition of iNOS and COX-2 expression via the down-regulation of NF-kappaB binding activity.

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