Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Expression, assay, and functional properties of RILP.

Tytuł:
Expression, assay, and functional properties of RILP.
Autorzy:
Colucci AM
Spinosa MR
Bucci C
Źródło:
Methods in enzymology [Methods Enzymol] 2005; Vol. 403, pp. 664-75.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: New York, Academic Press.
MeSH Terms:
Carrier Proteins/*physiology
Adaptor Proteins, Signal Transducing ; Base Sequence ; Carrier Proteins/genetics ; DNA Primers ; Genetic Vectors ; HeLa Cells ; Humans ; Lipoproteins, LDL/metabolism ; Microscopy, Confocal ; Microscopy, Fluorescence ; Organelles/metabolism
Substance Nomenclature:
0 (Adaptor Proteins, Signal Transducing)
0 (Carrier Proteins)
0 (DNA Primers)
0 (Lipoproteins, LDL)
0 (RILP protein, human)
Entry Date(s):
Date Created: 20060214 Date Completed: 20060425 Latest Revision: 20061115
Update Code:
20240104
DOI:
10.1016/S0076-6879(05)03057-0
PMID:
16473628
Czasopismo naukowe
Rab proteins are master regulators of vesicular membrane traffic of endocytic and exocytic pathways. They basically serve to recruit proteins and lipids required for vesicle formation, docking, and fusion. Each Rab protein is able to recruit one or more effectors, and, through the action of effectors, it drives its specific downstream functions. The Rab interacting lysosomal protein (RILP) is a common effector of Rab7 and Rab34, two Rab proteins implicated in the biogenesis of lysosomes. RILP is recruited onto late endosomal/lysosomal membranes by Rab7-GTP where it induces the recruitment of the dynein-dynactin motor complexes. Therefore, through the timed and selective dynein motor recruitment onto late endosomes and lysosomes, Rab7 and RILP control transport to endocytic degradative compartments. A similar role for Rab7 and RILP has been demonstrated also for phagosomes. Indeed, RILP recruits dynein-dynactin motors on Rab7-GTP-positive phagosomes and the recruitment not only displaces phagosomes centripetally, but also promotes the extension of phagosomal tubules toward late endocytic compartments. RILP is therefore a key protein for the biogenesis of lysosomes and phagolysosomes. This chapter describes how to express wild-type or mutated RILP in mammalian cells, and how to test the effects caused by RILP dysfunction. In particular, we report assays to monitor the interaction between RILP and Rab7, morphology and distribution of endosomes, and to measure degradation of endocytic markers.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies