[Retroviral-mediated gene transfer as an effective tool for the in vitro genetic transformation of chicken embryonic cells and production of transgenic chickens].

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Abstrakt

Tytuł:: [Retroviral-mediated gene transfer as an effective tool for the in vitro genetic transformation of chicken embryonic cells and production of transgenic chickens].
Autorzy:: Volkova NA
Zinov'eva NA
Volkova LV
Ernst LK
Źródło:: Genetika [Genetika] 2006 Jan; Vol. 42 (1), pp. 84-8.
Typ publikacji:: English Abstract; Journal Article
Język:: Russian
Imprint Name(s):: Original Publication: Moskva : Izdatelstvo Nauka
MeSH Terms:: Animals, Genetically Modified*
Gene Transfer Techniques*
Retroviridae*
Chick Embryo/*cytology
Chickens/*genetics
Animals ; Cells, Cultured ; Genetic Vectors ; In Vitro Techniques ; Organ Specificity ; Plasmids
Entry Date(s):: Date Created: 20060310 Date Completed: 20060421 Latest Revision: 20141120
Update Code:: 20240104
PMID:: 16523670
: Czasopismo naukowe

The data on the in vitro and in vivo (into embryonic disk) retroviral-mediated transfer of genetic information into chicken embryonic cells are presented. The estimated transformation frequency of the cultured target cells constituted 8 x 10(-4) to 5 x 10(-3). A transgenic rooster, carrying recombinant DNA in blood, heart, liver, and intestine cells, was obtained.

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