Alginic acid has anti-anaphylactic effects and inhibits inflammatory cytokine expression via suppression of nuclear factor-kappaB activation.

Szczegóły
Abstrakt

Tytuł:: Alginic acid has anti-anaphylactic effects and inhibits inflammatory cytokine expression via suppression of nuclear factor-kappaB activation.
Autorzy:: Jeong HJ; College of Pharmacy, VestibuloCochlear Research Center of Wonkwang University, Iksan Jeonbuk, Korea.
Lee SA
Moon PD
Na HJ
Park RK
Um JY
Kim HM
Hong SH
Źródło:: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology [Clin Exp Allergy] 2006 Jun; Vol. 36 (6), pp. 785-94.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Oxford : Blackwell Scientific Publications, c1989-
MeSH Terms:: Alginates/*pharmacology
Anti-Allergic Agents/*pharmacology
Cytokines/*analysis
Mast Cells/*metabolism
NF-kappa B/*metabolism
Animals ; Blotting, Western/methods ; Calcimycin ; Cell Line ; Cytokines/immunology ; Cytokines/metabolism ; Glucuronic Acid/pharmacology ; Hexuronic Acids/pharmacology ; Histamine Release ; Histidine Decarboxylase/analysis ; Histidine Decarboxylase/metabolism ; Ionophores ; Male ; Mast Cells/drug effects ; Mast Cells/immunology ; Models, Animal ; Passive Cutaneous Anaphylaxis ; Peritoneum ; Rats ; Rats, Wistar ; Skin Tests ; Tetradecanoylphorbol Acetate ; p-Methoxy-N-methylphenethylamine
Substance Nomenclature:: 0 (Alginates)
0 (Anti-Allergic Agents)
0 (Cytokines)
0 (Hexuronic Acids)
0 (Ionophores)
0 (NF-kappa B)
37H9VM9WZL (Calcimycin)
4091-50-3 (p-Methoxy-N-methylphenethylamine)
8A5D83Q4RW (Glucuronic Acid)
EC 4.1.1.22 (Histidine Decarboxylase)
NI40JAQ945 (Tetradecanoylphorbol Acetate)
Entry Date(s):: Date Created: 20060617 Date Completed: 20061027 Latest Revision: 20181201
Update Code:: 20240104
DOI:: 10.1111/j.1365-2222.2006.02508.x
PMID:: 16776680
: Czasopismo naukowe

Pełny tekst

Background: Alginic acid is comprised of complex polymerized polysaccharides, and can be chemically extracted from seaweed. Alginic acid has an inhibitory effect on histamine release, but its molecular mechanisms are not well understood.
Objective: To investigate the effect of alginic acid on the mast cell-mediated anaphylactic and inflammatory reaction using in vivo and in vitro models and elucidate its molecular mechanisms.
Materials and Method: The effect of alginic acid on an allergy model was analysed by anaphylaxis, a histidine decarboxylase (HDC) assay, and a histamine assay. Cytokine production was analysed by means of ELISA. Cytokine expression was analysed by means of RT-PCR, and Western blotting. Transcription factor activity was analysed by a luciferase assay and a transcription factor-enzyme linked immunoassay.
Results: Alginic acid dose dependently inhibited compound 48/80-induced systemic anaphylaxis with doses of 0.25-1 g/kg 1 h (P<0.01, n=6) and significantly inhibited passive cutaneous anaphylaxis by 54.8%. Alginic acid (0.01-1 microg/mL) inhibited histamine release from serum and peritoneal mast cells (P<0.01). All these effects were stronger than those of disodium cromoglycate (DSCG), the reference drug tested. Alginic acid also inhibited HDC expression and activity on the phorbol myristate acetate (PMA)+A23187-stimulated human mast cell line, HMC-1 cells. Moreover, alginic acid significantly inhibited the production of PMA+A23187-induced inflammatory cytokines, IL-1beta and TNF-alpha, but not that of IL-6 or IL-8. In activated HMC-1 cells, the expression level of nuclear factor (NF)-kappaB/Rel A protein increased in the nucleus, whereas the level of NF-kappaB/Rel A in the nucleus was decreased by alginic acid treatment. In addition, alginic acid (0.01 microg/mL) decreased the PMA+A23187-induced luciferase activity and DNA-binding activity.
Conclusion: The present results indicate that alginic acid has potent anti-anaphylactic and anti-inflammatory properties.

Informacja