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Tytuł:
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A new optical and nuclear dual-labeled imaging agent targeting interleukin 11 receptor alpha-chain.
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Autorzy:
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Wang W; Department of Radiology, Division of Molecular Imaging, Baylor College of Medicine, Houston, Texas 77030, USA. />Ke S
Kwon S
Yallampalli S
Cameron AG
Adams KE
Mawad ME
Sevick-Muraca EM
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Źródło:
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Bioconjugate chemistry [Bioconjug Chem] 2007 Mar-Apr; Vol. 18 (2), pp. 397-402. Date of Electronic Publication: 2007 Feb 22.
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Typ publikacji:
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Journal Article; Research Support, N.I.H., Extramural
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Język:
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English
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Imprint Name(s):
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Original Publication: Washington, DC : American Chemical Society, c1990-
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MeSH Terms:
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Fluorescent Dyes*/pharmacokinetics
Indium Radioisotopes*/pharmacokinetics
Peptide Fragments*/pharmacokinetics
Tomography, Emission-Computed, Single-Photon*
Breast Neoplasms/*diagnostic imaging
Receptors, Interleukin-11/*metabolism
Animals ; Breast Neoplasms/metabolism ; Cell Adhesion/drug effects ; Humans ; Light ; Mice ; Transplantation, Heterologous ; Tumor Cells, Cultured
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Grant Information:
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R01 EB003132 United States EB NIBIB NIH HHS
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Substance Nomenclature:
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0 (Fluorescent Dyes)
0 (Indium Radioisotopes)
0 (Peptide Fragments)
0 (Receptors, Interleukin-11)
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Entry Date(s):
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Date Created: 20070223 Date Completed: 20070503 Latest Revision: 20161124
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Update Code:
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20240104
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DOI:
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10.1021/bc0602679
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PMID:
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17313181
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Optical imaging has great potential for studying molecular recognitions both in vivo and in vitro, yet nuclear imaging is the most effective clinical molecular imaging modality. The combination of optical and nuclear imaging modalities may provide complementary information for improving diagnosis and management of diseases. In this study we developed an optical and nuclear dual-labeled imaging agent, 111In-DTPA-Bz-NH-SA-K(IR-783-S-Ph-CO)-c(CGRRAGGSC)NH2, called DLIA-IL11Ralpha. 111In-DTPA-Bz-NH-SA is the radiotracer moiety; a near-infrared dye IR-783-S-Ph-COOH serves as the fluorescent emitter; and the cyclic peptide c(CGRRAGGSC), which is known to target interleukin 11 receptor alpha-chain (IL-11Ralpha), delivers the desired imaging agent to its target. Experiments revealed that the cyclic peptide c(CGRRAGGSC) continued to possess the targeting capability to IL-11Ralpha after the conjugation of the optical and nuclear tracers. Furthermore, the presence of the metal isotope chelator did not cause quenching of fluorescence emission. The cross validation and direct comparison of optical and nuclear imaging of a tumor was achieved using a single injection, and the preliminary results show the conjugate has tumor targeting capabilities in vivo.
