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Tytuł pozycji:

Comethylation of p16 and MGMT genes in colorectal carcinoma: correlation with clinicopathological features and prognostic value.

Tytuł:
Comethylation of p16 and MGMT genes in colorectal carcinoma: correlation with clinicopathological features and prognostic value.
Autorzy:
Krtolica K; Laboratory for Radiobiology and Molecular Genetics, Institute of Nuclear Sciences Vinca, P. O. BOX 522, Belgrade, Serbia. />Krajnovic M
Usaj-Knezevic S
Babic D
Jovanovic D
Dimitrijevic B
Źródło:
World journal of gastroenterology [World J Gastroenterol] 2007 Feb 28; Vol. 13 (8), pp. 1187-94.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: 2014- : Pleasanton, CA : Baishideng Publishing Group
Original Publication: Beijing : WJG Press, c1998-
MeSH Terms:
Genes, p16*
Genes, ras*
Carcinoma/*genetics
Colorectal Neoplasms/*genetics
DNA Modification Methylases/*genetics
DNA Repair Enzymes/*genetics
Tumor Suppressor Proteins/*genetics
Adult ; Age Factors ; Aged ; Cadherins/metabolism ; Carcinoma/mortality ; Carcinoma/pathology ; Cell Proliferation ; Colon/pathology ; Colorectal Neoplasms/mortality ; Colorectal Neoplasms/pathology ; DNA Methylation ; Female ; Humans ; Hyaluronan Receptors/metabolism ; Immunohistochemistry ; Laminin/metabolism ; Male ; Middle Aged ; Point Mutation ; Promoter Regions, Genetic ; Rectum/pathology ; Survival Analysis
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Substance Nomenclature:
0 (CD44 protein, human)
0 (Cadherins)
0 (Hyaluronan Receptors)
0 (Laminin)
0 (Tumor Suppressor Proteins)
EC 2.1.1.- (DNA Modification Methylases)
EC 2.1.1.63 (MGMT protein, human)
EC 6.5.1.- (DNA Repair Enzymes)
Entry Date(s):
Date Created: 20070425 Date Completed: 20070717 Latest Revision: 20190430
Update Code:
20240104
PubMed Central ID:
PMC4146992
DOI:
10.3748/wjg.v13.i8.1187
PMID:
17451198
Czasopismo naukowe
Aim: To investigate the significance of p16 and O(6)-methylguanine-DNA methyltransferase (MGMT) genes promoter hypermethylation and K-ras mutations on colorectal tumorigenesis and progression.
Methods: p16 and MGMT methylation status was examined on 47 tumor samples, and K-ras mutational status was examined on 85 tumor samples. For methylation analysis, a methylation specific PCR (MS-PCR) method was used.
Results: p16 and MGMT promoter methylation was found in 51% (24/47) and 43% (20/47) of CRCs, respectively, and the K-ras mutation was found in 44% (37/85) of CRCs. Comethylation of p16 and MGMT genes was significantly associated with lower aggressiveness of the disease within a two-year period of observation. Only 27% of patients with simultaneous p16 and MGMT methylation showed the detectable occurrence of metastasis and/or death, compared to 67% of patients without double methylation or with no methylation (3/11 vs 22/33, P < 0.05, chi(2)-test). In addition, p16 and MGMT comethylation showed a trend toward an association with longer survival in patients with CRCs (35.5 +/- 6.0 mo vs 23.1 +/- 3.2 mo, P = 0.072, Log-rank test). Progression of the disease within a two-year period was observed in 66% of patients carrying the K-ras mutation, compared to only 19% of patients with wild type K-ras (29/44 vs 7/37, P < 0.001, chi(2)-test). The presence of the K-ras mutation significantly correlated to shortened overall survival (20.0 +/- 1.9 mo vs 37.0 +/- 1.8 mo, P < 0.001, Log-rank test). The comethylation of p16 and MGMT genes was significantly associated with lower aggressiveness of the disease even when K-ras mutations were included in the analysis as an independent variable.
Conclusion: Our data suggest that comethylation of promoters of p16 and MGMT genes could have a prognostic value in patients with CRC. Specifically, concurrent methylation of both genes correlates with better prognosis.

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