Monomeric red fluorescent protein as a reporter for macromolecular localization in Streptomyces coelicolor.

Szczegóły
Abstrakt

Tytuł:: Monomeric red fluorescent protein as a reporter for macromolecular localization in Streptomyces coelicolor.
Autorzy:: Nguyen KD; Department of Biochemistry and Biomedical Sciences, McMaster University, Health Sciences Centre, 1200 Main Street West, Hamilton, Ont., Canada L8N 3Z5.
Au-Young SH
Nodwell JR
Źródło:: Plasmid [Plasmid] 2007 Sep; Vol. 58 (2), pp. 167-73. Date of Electronic Publication: 2007 Jun 04.
Typ publikacji:: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: New York, Academic Press.
MeSH Terms:: Genes, Reporter*
Bacterial Proteins/*metabolism
Luminescent Proteins/*genetics
Streptomyces coelicolor/*genetics
Streptomyces coelicolor/*growth & development
Fluorescent Dyes/metabolism ; Gene Fusion ; Genes, Bacterial ; Genetic Vectors ; Luminescent Proteins/metabolism ; Microscopy, Fluorescence ; Restriction Mapping ; Spores, Bacterial/growth & development ; Streptomyces coelicolor/metabolism ; Time Factors ; Red Fluorescent Protein
Substance Nomenclature:: 0 (Bacterial Proteins)
0 (Fluorescent Dyes)
0 (Luminescent Proteins)
Entry Date(s):: Date Created: 20070605 Date Completed: 20071206 Latest Revision: 20231213
Update Code:: 20240104
DOI:: 10.1016/j.plasmid.2007.03.005
PMID:: 17544507
: Czasopismo naukowe

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The enhanced green fluorescent protein (eGFP) is widely used to investigate cell type specific gene expression and protein localization in the filamentous streptomycetes. To broaden the scope of cell biological investigation in these organisms, we have adapted shuttle vectors for the construction of gene fusions to the monomeric red fluorescent protein (mRFP1) and have tested them in Streptomyces coelicolor. Using fusions of mRFP1 to the cell division proteins DivIVA and FtsZ, we show that mRFP1 is comparable to eGFP for cell biological research in this organism and suggest that this paves the way for the future use of two-color imaging and FRET.

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