Identification of essential genes in Staphylococcus aureus by construction and screening of conditional mutant library.

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Abstrakt

Tytuł:: Identification of essential genes in Staphylococcus aureus by construction and screening of conditional mutant library.
Autorzy:: Yin D; Aastrom Biosciences Inc., Ann Arbor, MI, USA.
Ji Y
Źródło:: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2008; Vol. 416, pp. 297-305.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:: Gene Targeting*
Genes, Essential/*physiology
RNA, Antisense/*genetics
Staphylococcus aureus/*genetics
Cloning, Molecular ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; RNA, Antisense/metabolism ; Staphylococcus aureus/metabolism
Substance Nomenclature:: 0 (RNA, Antisense)
Entry Date(s):: Date Created: 20080409 Date Completed: 20080620 Latest Revision: 20080408
Update Code:: 20240104
DOI:: 10.1007/978-1-59745-321-9_19
PMID:: 18392975
: Czasopismo naukowe

Antisense RNA technology has been used effectively to downregulate gene expression in a variety of bacterial systems. Regulated antisense RNA strategy provides an important approach to identify and characterize essential genes critical to bacterial growth in vitro and in vivo. This strategy allows selective genes to be turned on or off and to be expressed at certain levels. The availability of the Staphylococcus aureus (S. aureus) genome sequence makes it feasible to generate a gene-specific antisense RNA library. The combination of regulated antisense RNA technology and the gene-specific antisense RNA library allows for genome-wide analyses of functions of staphylococcal gene products for growth in culture and survival during infection.

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