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Tytuł pozycji:

A stereological method for estimating the total length and size of myelinated fibers in rat cerebral cortex.

Tytuł :
A stereological method for estimating the total length and size of myelinated fibers in rat cerebral cortex.
Autorzy :
Zhang W; Institute for Basic Medical Sciences, Chongqing Medical University, Chongqing, PR China.
Li C
Yang S
Xu C
Wang W
Nyengaard JR
Tang Y
Pokaż więcej
Źródło :
Journal of neuroscience methods [J Neurosci Methods] 2008 Jul 15; Vol. 172 (1), pp. 21-6. Date of Electronic Publication: 2008 Apr 14.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
MeSH Terms :
Stereotaxic Techniques*
Cerebral Cortex/*cytology
Nerve Fibers, Myelinated/*physiology
Animals ; Biometry ; Cell Size ; Female ; Microscopy, Electron, Transmission/methods ; Nerve Fibers, Myelinated/ultrastructure ; Rats ; Rats, Long-Evans
Entry Date(s) :
Date Created: 20080527 Date Completed: 20080919 Latest Revision: 20080603
Update Code :
20210210
DOI :
10.1016/j.jneumeth.2008.04.005
PMID :
18501432
Czasopismo naukowe
An efficient and unbiased stereological technique to estimate the total length and total volume of the myelinated fibers in rat cortex is described. Unbiased sampling and measuring principles enable to obtain an unbiased estimation of the true value. In the present design, six tissue blocks were obtained from the entire rat brain cortex in a uniform random fashion. Isotropic, uniform random (IUR) sections were ensured by the use of the isector technique. One section with the thickness of 60 nm was cut from the center of each epon block using an ultramicrotome. Five fields of vision were photographed using electron microscopy. The length density of the myelinated fibers in the cortex was estimated using an unbiased counting frame. The volume density of the myelinated fibers in the cortex was estimated with point counting. The total length and total volume of the myelinated fibers in the cortex were estimated by multiplying the volume of the cortex, obtained using the Cavalieri principle and the length density and volume density of the fibers in the cortex. The sampling variance introduced by the stereological estimation procedure was a small fraction of the observed total variance indicating an efficient sampling protocol.

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