Capillary electrophoresis with gold nanoparticles enhanced electrochemiluminescence for the detection of roxithromycin.

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Abstrakt

Tytuł:: Capillary electrophoresis with gold nanoparticles enhanced electrochemiluminescence for the detection of roxithromycin.
Autorzy:: Wang J; Department of Chemistry, Nanchang University, Nanchang 330031, China. />Yang Z
Wang X
Yang N
Źródło:: Talanta [Talanta] 2008 Jun 30; Vol. 76 (1), pp. 85-90. Date of Electronic Publication: 2008 Feb 19.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
MeSH Terms:: Luminescence*
Electrophoresis, Capillary/*methods
Gold/*chemistry
Metal Nanoparticles/*chemistry
Roxithromycin/*analysis
Roxithromycin/*chemistry
Anti-Bacterial Agents/analysis ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/urine ; Electrochemistry ; Organometallic Compounds/chemistry ; Roxithromycin/urine ; Sensitivity and Specificity
Substance Nomenclature:: 0 (Anti-Bacterial Agents)
0 (Organometallic Compounds)
0 (tris(2,2'-bipyridyl)ruthenium(II))
21KOF230FA (Roxithromycin)
7440-57-5 (Gold)
Entry Date(s):: Date Created: 20080701 Date Completed: 20080924 Latest Revision: 20131121
Update Code:: 20240104
DOI:: 10.1016/j.talanta.2008.02.006
PMID:: 18585245
: Czasopismo naukowe

Tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy)(3)(2+))-roxithromycin based electrochemiluminescence (ECL) was enhanced greatly by gold nanoparticles 10 nm in diameter. Capillary electrophoresis (CE) was coupled with the resultant ECL system as a detector for roxithromycin. This ECL emission is explained by the coreactant mechanism where roxithromycin behaves as a coreactant to generate strong reducing species and gold nanoparticles act as "floating nanoelectrodes". The reaction of Ru(bpy)(3)(3+) with the generated strong reducing species on the Pt working electrode as well as on "floating nanoelectrodes" releases Ru(bpy)(3)(2+*), resulting in enhancement of ECL emission. The selectivity of this detection system towards roxithromycin was examined by CE. Under the optimized conditions, the intensity of ECL emission varies linearly with the concentration of roxithromycin from 24 nM to 0.24 mM. The detection limit is 8.4 nM, while without adding gold nanoparticles it is only 84 nM. The detection of roxithromycin in pharmaceutical and urine samples was also performed by the proposed CE-ECL method.

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