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Tytuł:
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Potential chemoprotective effect of melatonin in cyclophosphamide- and cisplatin-induced testicular damage in rats.
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Autorzy:
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Ilbey YO; Department of Urology, Bezm-i Alem Valide Sultan Vakıf Gureba Research and Education Hospital, Istanbul, Turkey. Electronic address: .
Ozbek E; Department of Urology, Bezm-i Alem Valide Sultan Vakıf Gureba Research and Education Hospital, Istanbul, Turkey.
Simsek A; Department of Urology, Bezm-i Alem Valide Sultan Vakıf Gureba Research and Education Hospital, Istanbul, Turkey.
Otunctemur A; Department of Urology, Bezm-i Alem Valide Sultan Vakıf Gureba Research and Education Hospital, Istanbul, Turkey.
Cekmen M; Department of Biochemistry, Kocaeli University, Istanbul, Turkey.
Somay A; Department of Pathology, Bezm-i Alem Valide Sultan Vakıf Gureba Research and Education Hospital, Istanbul, Turkey.
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Źródło:
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Fertility and sterility [Fertil Steril] 2009 Sep; Vol. 92 (3), pp. 1124-1132. Date of Electronic Publication: 2008 Oct 01.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: New York. NY : Elsevier for the American Society for Reproductive Medicine
Original Publication: New York, Hoeber.
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MeSH Terms:
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Antioxidants/*pharmacology
Cisplatin/*adverse effects
Cyclophosphamide/*adverse effects
Melatonin/*pharmacology
Testis/*drug effects
Testis/*pathology
Animals ; Antioxidants/administration & dosage ; Cisplatin/administration & dosage ; Cisplatin/pharmacology ; Cyclophosphamide/administration & dosage ; Cyclophosphamide/pharmacology ; Fibrosis ; Glutathione/metabolism ; Glutathione Peroxidase/metabolism ; Injections, Intraperitoneal ; Lipid Peroxidation/drug effects ; Male ; Malondialdehyde/metabolism ; Melatonin/administration & dosage ; Models, Animal ; Rats ; Rats, Wistar ; Spermatogenesis/drug effects ; Testis/metabolism ; Testosterone/blood
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Substance Nomenclature:
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0 (Antioxidants)
3XMK78S47O (Testosterone)
4Y8F71G49Q (Malondialdehyde)
8N3DW7272P (Cyclophosphamide)
EC 1.11.1.9 (Glutathione Peroxidase)
GAN16C9B8O (Glutathione)
JL5DK93RCL (Melatonin)
Q20Q21Q62J (Cisplatin)
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Entry Date(s):
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Date Created: 20081003 Date Completed: 20090923 Latest Revision: 20220408
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Update Code:
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20240104
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DOI:
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10.1016/j.fertnstert.2008.07.1758
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PMID:
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18829000
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Objective: To evaluate the effect of melatonin on cyclophosphamide (CP)- and cisplatin-induced testicular toxicity with use of sperm parameters and biochemical and histopathologic approaches.
Design: Experimental study.
Setting: Vakif Gureba Hospital, Istanbul, Turkey.
Animals: Six-week-old adult male Wistar albino rats (N = 48).
Intervention(s): Cyclophosphamide was administered to rats by gavage at a single dose of 100 mg/kg body weight, only once. Cisplatin was injected intraperitoneally at single doses of 7 mg/kg/d for five consecutive days. Melatonin was both administered separately and coadministered with CP and cisplatin intraperitoneally at a dose of 10 mg/kg body weight.
Main Outcome Measure(s): Body and testicular weight, epididymal sperm characteristics, plasma T, and histopathologic structure of the testicular tissue were determined. Malondialdehyde (MDA) and reduced glutathione (GSH) levels and glutathione peroxidase (GSH-Px) activity were assessed in testicular tissue.
Result(s): Body and testicular weight, epididymal sperm count, motility and morphology, plasma T levels, the activities of GSH-Px, and GSH levels were significantly decreased whereas the level of MDA was significantly increased in rats of the CP and cisplatin group. Melatonin treatment increased GSH levels and GSH-Px activity, decreased MDA level in testicular tissue, and increased plasma T level. Cyclophosphamide and cisplatin caused irregular seminiferous tubules, reduction of seminiferous epithelial layers, significant maturation arrest, and perivascular fibrosis. Melatonin significantly improved histopathologic changes.
Conclusion(s): Melatonin may prevent CP- and cisplatin-induced testicular damage.
