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Tytuł:
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Determination of plasma heparin by micellar electrokinetic capillary chromatography.
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Autorzy:
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Zhou XM; Department of Medical Laboratory, NanFang Hospital, Guangzhou 510515, People's Republic of China.
Liu JW
Zhang ME
Chen SJ
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Źródło:
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Talanta [Talanta] 1998 Aug; Vol. 46 (4), pp. 757-60.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
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Entry Date(s):
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Date Created: 20081031 Date Completed: 20121002 Latest Revision: 20190729
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Update Code:
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20240104
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DOI:
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10.1016/s0039-9140(98)00040-x
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PMID:
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18967202
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The micellar electrokinetic capillary chromatography (MECC) method is reported for the separation of heparin, and for the possibility of direct determination of free heparin in plasma. The conditions for MECC were: pH 8.5, 25 mM sodium dodecyl sulfate (SDS), 25 mM borate buffer, with a 30 cmx50 mum ID fused-silica capillary. The sample was detected with a UV-detector at 270 nm with heparin as external standard. The recovery rate was 95.6-98.7%. This method was linear in the range 80-7000 U l(-1). The within-run and between-run relative standard deviations were lower than 3.1 and 4.5%, respectively. It is suggested that this MECC method may be used to determine blood samples containing high levels of heparin.