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Tytuł:
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Non-blinking and photostable upconverted luminescence from single lanthanide-doped nanocrystals.
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Autorzy:
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Wu S; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Han G
Milliron DJ
Aloni S
Altoe V
Talapin DV
Cohen BE
Schuck PJ
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Źródło:
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Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2009 Jul 07; Vol. 106 (27), pp. 10917-21. Date of Electronic Publication: 2009 Jun 18.
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Typ publikacji:
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Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
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Język:
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English
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Imprint Name(s):
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Original Publication: Washington, DC : National Academy of Sciences
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MeSH Terms:
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Luminescence*
Lanthanoid Series Elements/*chemistry
Nanoparticles/*chemistry
Animals ; Cell Survival ; Fibroblasts/cytology ; Membranes, Artificial ; Mice ; NIH 3T3 Cells ; Nanoparticles/ultrastructure ; Silicon Compounds/chemistry ; Solubility ; Water
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References:
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Substance Nomenclature:
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0 (Lanthanoid Series Elements)
0 (Membranes, Artificial)
0 (Silicon Compounds)
059QF0KO0R (Water)
QHB8T06IDK (silicon nitride)
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Entry Date(s):
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Date Created: 20090623 Date Completed: 20090727 Latest Revision: 20211020
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Update Code:
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20240104
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PubMed Central ID:
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PMC2698891
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DOI:
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10.1073/pnas.0904792106
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PMID:
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19541601
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The development of probes for single-molecule imaging has dramatically facilitated the study of individual molecules in cells and other complex environments. Single-molecule probes ideally exhibit good brightness, uninterrupted emission, resistance to photobleaching, and minimal spectral overlap with cellular autofluorescence. However, most single-molecule probes are imperfect in several of these aspects, and none have been shown to possess all of these characteristics. Here we show that individual lanthanide-doped upconverting nanoparticles (UCNPs)--specifically, hexagonal phase NaYF(4) (beta-NaYF(4)) nanocrystals with multiple Yb(3+) and Er(3+) dopants--emit bright anti-Stokes visible upconverted luminescence with exceptional photostability when excited by a 980-nm continuous wave laser. Individual UCNPs exhibit no on/off emission behavior, or "blinking," down to the millisecond timescale, and no loss of intensity following an hour of continuous excitation. Amphiphilic polymer coatings permit the transfer of hydrophobic UCNPs into water, resulting in individual water-soluble nanoparticles with undiminished photophysical characteristics. These UCNPs are endocytosed by cells and show strong upconverted luminescence, with no measurable anti-Stokes background autofluorescence, suggesting that UCNPs are ideally suited for single-molecule imaging experiments.