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Tytuł:
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Efficacy of butyrate analogues in HT-29 cancer cells.
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Autorzy:
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Ooi CC; CSIRO Preventative Health Flagship, Sansom Institute, University of South Australia, Adelaide, South Australia, Australia.
Good NM
Williams DB
Lewanowitsch T
Cosgrove LJ
Lockett TJ
Head RJ
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Źródło:
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Clinical and experimental pharmacology & physiology [Clin Exp Pharmacol Physiol] 2010 Apr; Vol. 37 (4), pp. 482-9. Date of Electronic Publication: 2009 Nov 23.
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Typ publikacji:
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Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: Oxford, England : Wiley-Blackwell
Original Publication: Oxford, Blackwell Scientific Publications.
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MeSH Terms:
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Antineoplastic Agents/*pharmacology
Apoptosis/*drug effects
Butyrates/*pharmacology
Cell Proliferation/*drug effects
Histone Deacetylase Inhibitors/*pharmacology
Antineoplastic Agents/chemistry ; Butyrates/chemistry ; Butyric Acid/pharmacology ; Dose-Response Relationship, Drug ; Drug Screening Assays, Antitumor ; HT29 Cells ; Histone Deacetylase Inhibitors/chemistry ; Histone Deacetylases/metabolism ; Humans ; Lactate Dehydrogenases/metabolism ; Statistics as Topic ; Structure-Activity Relationship
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Substance Nomenclature:
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0 (Antineoplastic Agents)
0 (Butyrates)
0 (Histone Deacetylase Inhibitors)
107-92-6 (Butyric Acid)
595CFD21KA (4-benzoylbutyric acid)
EC 1.1.- (Lactate Dehydrogenases)
EC 3.5.1.98 (Histone Deacetylases)
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Entry Date(s):
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Date Created: 20091126 Date Completed: 20100806 Latest Revision: 20190111
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Update Code:
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20240104
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DOI:
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10.1111/j.1440-1681.2009.05335.x
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PMID:
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19930426
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1. Butyrate is a well known product of starch fermentation by colonic bacteria and is of interest owing to its ability to induce in vitro apoptosis and cell differentiation, as well as to inhibit cell growth in colorectal and other cancer cells. Synthetic analogues of butyrate may also possess cellular activities in a variety of cultured cells. The aim of the present study was to evaluate the effects of butyrate analogues on apoptosis, proliferation and histone deacetylase (HDAC) activity in HT-29 colorectal cancer cells. In addition, the effects of these analogues on lactate dehydrogenase leakage, as a measure of non-specific cytotoxicity, were evaluated in HT-29 cells. 2. Of the 26 analogues examined, four (propionate, 4-benzoylbutyrate, 4-(4-aminophenyl)butyrate and benzyloxyacetate) exhibited comparable effects to butyrate. Interestingly, no activity was noted for compounds carrying amino, hydroxyl or methyl substitutions at the 2-, 3- or 4-position of the aliphatic moiety of butyrate. 3. In conclusion, chemical changes to the structure of butyrate can significantly modify the biological activity assayed in HT-29 colorectal cancer cells in vitro.