Amplification and deletion of a nod-nif region in the symbiotic plasmid of Rhizobium phaseoli.

Szczegóły
Abstrakt

Tytuł:: Amplification and deletion of a nod-nif region in the symbiotic plasmid of Rhizobium phaseoli.
Autorzy:: Romero D; Departamento de Genética Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
Brom S
Martínez-Salazar J
Girard ML
Palacios R
Dávila G
Źródło:: Journal of bacteriology [J Bacteriol] 1991 Apr; Vol. 173 (8), pp. 2435-41.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Język:: English
Imprint Name(s):: Original Publication: Washington, DC : American Society for Microbiology
MeSH Terms:: Gene Rearrangement*
Genes, Bacterial*
Nitrogen Fixation/*genetics
Rhizobium/*genetics
Blotting, Southern ; Chromosome Deletion ; Chromosome Mapping ; Escherichia coli/genetics ; Gene Amplification ; Plasmids/genetics ; Transformation, Bacterial
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Gene Symbol:: lacZ; nifD; nifH; nifK; nod; sacRB
Entry Date(s):: Date Created: 19910401 Date Completed: 19910514 Latest Revision: 20190508
Update Code:: 20240104
PubMed Central ID:: PMC207805
DOI:: 10.1128/jb.173.8.2435-2441.1991
PMID:: 2013567
: Czasopismo naukowe

One remarkable characteristic of the genomes of some Rhizobium species is the frequent occurrence of rearrangements. In some instances these rearrangements alter the symbiotic properties of the strains. However, no detailed molecular mechanisms have been proposed for the generation of these rearrangements. To understand the mechanisms involved in the formation of rearrangements in the genome of Rhizobium phaseoli, we have designed a system which allows the positive selection for amplification and deletion events. We have applied this system to investigate the stability of the symbiotic plasmid of R. phaseoli. High-frequency amplification events were detected which increase the copy number of a 120-kb region carrying nodulation and nitrogen fixation genes two to eight times. Deletion events that affect the same region were also found, albeit at a lower frequency. Both kinds of rearrangements are generated by recombination between reiterated nitrogenase (nifHDK) operons flanking the 120-kb region.

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