-
Tytuł:
-
[Construction of DNA vaccine pcDNA3.1(+)/tetraspanin 2-A against Schistosoma japonicum and its immune-protective effect in mice].
-
Autorzy:
-
Zhang P; Department of Microbiology and Parasitology, Anhui Medical University, Hefei 230032, China.
Zhang WN
Ren CP
Liu M
Shen JJ
-
Źródło:
-
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases [Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi] 2009 Dec; Vol. 27 (6), pp. 534-6.
-
Typ publikacji:
-
English Abstract; Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
Chinese
-
Imprint Name(s):
-
Publication: Shanghai : Institute Of Parasitic Diseases, Chinese Academy Of Preventive Medicine
Original Publication: Shanghai Shi : Zhongguo yu fang yi xue ke xue yuan Ji sheng chong bing yan jiu suo, 1987-
-
MeSH Terms:
-
Antigens, Helminth/*immunology
Membrane Proteins/*immunology
Schistosoma japonicum/*immunology
Schistosomiasis japonica/*prevention & control
Vaccines, DNA/*immunology
Animals ; Antibodies, Helminth/immunology ; Mice ; Mice, Inbred BALB C ; Plasmids
-
Substance Nomenclature:
-
0 (Antibodies, Helminth)
0 (Antigens, Helminth)
0 (Membrane Proteins)
0 (Vaccines, DNA)
-
Entry Date(s):
-
Date Created: 20100318 Date Completed: 20101203 Latest Revision: 20100317
-
Update Code:
-
20240104
-
PMID:
-
20232641
-
Tetraspanin 2-A (SjTsp2-A) gene was amplified by PCR. pcDNA3.1(+)/SjTsp2-A recombinant plasmids were constructed and transformed into E. coli DH5alpha. Twenty four BALB/c mice were randomly divided into pcDNA3.1(+)/ -SjTsp2-A group (A), pcDNA3.1(+)/SjGST group (B) and pcDNA3.1(+) group (C). Each mouse was injected through musculus quadriceps femoris by three times (two weeks interval) respectively with 100 microg pcDNA3.1(+)/SjTsp2-A, pcDNA3.1 (+)/SjGST, or pcDNA3.1(+). At two weeks after the final inoculation, mice were each challenged by 40 +/- 2 cercariae of S. japonicum. Forty-five days after infection, all mice were sacrificed, the number of worms collected and eggs in liver tissue was counted. Anti-pcDNA3.1(+)/SjTsp2-A antibody was detected by ELISA and protein expression in quadriceps muscle by immunohistochemical staining. The worm reduction rate (44.4%) and egg reduction rate (28.4%) of group A was higher than those of group B and C (P < 0.05), but no significant difference between groups B (3.9%, 19.3%) and C. Higher antibody titer (1:25 600) was detected in sera of group A. Immunohistochemistry analysis showed an expression of specific antigens in quadriceps muscles of groups A and B. The DNA candidate vaccine induces partial protective immunity against S japonicum in BALB/c mice.