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Tytuł pozycji:

A methodological reappraisal of total and high molecular weight adiponectin determination in human peripheral circulation: comparison of four immunometric assays.

Tytuł:
A methodological reappraisal of total and high molecular weight adiponectin determination in human peripheral circulation: comparison of four immunometric assays.
Autorzy:
Caselli C; Laboratory of Cardiovascular Biochemistry, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche, Pisa, Italy.
Melaiu O
Maltinti M
Del Ry S
Cabiati M
Prescimone T
Neglia D
Giannessi D
Źródło:
Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2010 Apr; Vol. 48 (4), pp. 561-8.
Typ publikacji:
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: Berlin ; New York : Walter De Gruyter, c1998-
MeSH Terms:
Adiponectin/*blood
Enzyme-Linked Immunosorbent Assay/*methods
Adiponectin/chemistry ; Adult ; Aged ; Heart Diseases/diagnosis ; Humans ; Male ; Middle Aged ; Molecular Weight ; Protein Multimerization ; ROC Curve ; Reagent Kits, Diagnostic
Substance Nomenclature:
0 (Adiponectin)
0 (Reagent Kits, Diagnostic)
Entry Date(s):
Date Created: 20100320 Date Completed: 20100615 Latest Revision: 20161018
Update Code:
20240104
DOI:
10.1515/CCLM.2010.104
PMID:
20298133
Czasopismo naukowe
Background: Adiponectin, present in different multimeric forms in circulation, is increasingly used in clinical settings as a cardiometabolic marker. The development of several commercially available enzyme-linked immunosorbent assays (ELISA) has allowed for the widespread measurement of adiponectin in research as well as in clinical practice. The comparative performance of these assays is thus an issue of major relevance.
Methods: The analytical performance of four different ELISAs (LINCO, B-Bridge, SPIbio and ALPCO) was evaluated. Samples from 102 cardiac patients and 40 healthy subjects were tested using LINCO and ALPCO. The latter is able to measure the concentrations of multimers. For the multimer assay, an error propagation study was performed. A subset of subjects was tested by all four ELISAs for comparison purposes.
Results: Bland-Altman plots revealed differences between the results of the four ELISAs, mainly for ALPCO. However, a strong correlation between the different ELISAs (ALPCO, r=0.83; B-Bridge, r=0.98; SPIbio, r=0.91 vs. LINCO) was observed. Total adiponectin measured by LINCO showed a better association with cardiac disease [receiver-operating characteristic (ROC) curves] than total and high molecular weight adiponectin measured by ALPCO.
Conclusions: The results of this study contribute to a better understanding of the methodological features of the several ELISAs, and help in the evaluation and comparison of the relative results.

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