Singlet oxygen scavenging activity of plastoquinol in photosystem II of higher plants: electron paramagnetic resonance spin-trapping study.

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Tytuł:: Singlet oxygen scavenging activity of plastoquinol in photosystem II of higher plants: electron paramagnetic resonance spin-trapping study.
Autorzy:: Yadav DK; Laboratory of Biophysics, Department of Experimental Physics, Faculty of Science, Palacký University, tr. 17. Listopadu 12, Olomouc, Czech Republic.
Kruk J
Sinha RK
Pospíšil P
Źródło:: Biochimica et biophysica acta [Biochim Biophys Acta] 2010 Nov; Vol. 1797 (11), pp. 1807-11. Date of Electronic Publication: 2010 Jul 15.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Amsterdam : Elsevier Pub. Co.
MeSH Terms:: Electron Spin Resonance Spectroscopy*
Free Radical Scavengers/*pharmacology
Photosystem II Protein Complex/*chemistry
Plastoquinone/*analogs & derivatives
Singlet Oxygen/*chemistry
Spinacia oleracea/*chemistry
Cell Membrane/drug effects ; Cell Membrane/metabolism ; Photosystem II Protein Complex/metabolism ; Plastoquinone/chemistry ; Spin Trapping
Substance Nomenclature:: 0 (Free Radical Scavengers)
0 (Photosystem II Protein Complex)
17778-80-2 (Singlet Oxygen)
3819-09-8 (plastoquinol)
OAC30J69CN (Plastoquinone)
Entry Date(s):: Date Created: 20100720 Date Completed: 20101116 Latest Revision: 20161126
Update Code:: 20240104
DOI:: 10.1016/j.bbabio.2010.07.003
PMID:: 20637718
: Czasopismo naukowe

Singlet oxygen (¹O₂) scavenging activity of plastoquinol in photosystem II (PSII) of higher plants was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that illumination of spinach PSII membranes deprived of intrinsic plastoquinone results in ¹O₂ formation, as monitored by TEMPONE EPR signal. Interestingly, the addition of exogenous plastoquinol (PQH₂-1) to PQ-depleted PSII membranes significantly suppressed TEMPONE EPR signal. The presence of exogenous plastoquinols with a different side-chain length (PQH₂-n, n isoprenoid units in the side chain) caused a similar extent of ¹O₂ scavenging activity. These observations reveal that plastoquinol exogenously added to PQ-depleted PSII membranes serves as efficient scavenger of ¹O₂.
(Copyright © 2010 Elsevier B.V. All rights reserved.)

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