[Effect of Bufalin on proliferation and apoptosis of human non-small cell lung cancer A549 cell].

Tytuł:: [Effect of Bufalin on proliferation and apoptosis of human non-small cell lung cancer A549 cell].
Autorzy:: Zhu Z; Department of Oncology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Yu Y
Wang K
Li E
Liu Y
Liu Y
Źródło:: Zhongguo fei ai za zhi = Chinese journal of lung cancer [Zhongguo Fei Ai Za Zhi] 2010 Sep; Vol. 13 (9), pp. 841-5.
Typ publikacji:: English Abstract; Journal Article; Research Support, Non-U.S. Gov't; Retracted Publication
Język:: Chinese
Imprint Name(s):: Publication: 2008- : Tianjin Shi : Zhongguo fei ai za zhi bian ji wei yuan hui
Original Publication: Chendu Shi : Zhongguo fei ai za zhi bian ji wei yuan hui
MeSH Terms:: Apoptosis/*drug effects
Bufanolides/*pharmacology
Carcinoma, Non-Small-Cell Lung/*drug therapy
Carcinoma, Non-Small-Cell Lung/*physiopathology
Cell Proliferation/*drug effects
Animals ; Bufonidae ; Carcinoma, Non-Small-Cell Lung/enzymology ; Caspase 3/genetics ; Caspase 3/metabolism ; Cell Cycle/drug effects ; Cell Line, Tumor ; Humans ; Lung Neoplasms/drug therapy ; Lung Neoplasms/enzymology ; Lung Neoplasms/physiopathology
References:: World J Gastroenterol. 2007 Jun 28;13(24):3374-9. (PMID: 17659679)
Anticancer Drugs. 2009 Jan;20(1):59-64. (PMID: 19343001)
J Steroid Biochem Mol Biol. 2009 Apr;114(3-5):144-51. (PMID: 19429444)
Mol Hum Reprod. 2005 Nov;11(11):817-23. (PMID: 16390854)
J Biol Chem. 2001 Feb 2;276(5):3238-46. (PMID: 11024045)
Semin Respir Crit Care Med. 2004;25 Suppl 1:3-10. (PMID: 16088515)
Biotechnol Lett. 2009 Apr;31(4):487-94. (PMID: 19039527)
Mol Cell Biochem. 2009 Jan;320(1-2):133-40. (PMID: 18827979)
Anticancer Res. 2007 Jan-Feb;27(1A):245-9. (PMID: 17352239)
Oncogene. 2003 Nov 13;22(51):8330-6. (PMID: 14614456)
Substance Nomenclature:: 0 (Bufanolides)
EC 3.4.22.- (Caspase 3)
U549S98QLW (bufalin)
Entry Date(s):: Date Created: 20100916 Date Completed: 20101221 Latest Revision: 20211020
Update Code:: 20240104
PubMed Central ID:: PMC7969378
DOI:: 10.3779/j.issn.1009-3419.2010.09.01
PMID:: 20840810
: Czasopismo naukowe

Full Text Finder

Background and Objective: Bufalin, a component of the Chinese medicine chan'su, induces apoptosis in various human tumor cell lines. The aim of this study is to investigate the mechanism of anti-proliferation and the induction of apoptosis of Bufalin on non-small cell lung cancer cell.
Methods: The effect of Bufalin on A549 cell proliferation was measured using MTT assay; the morphological change was observed with Wright-Giemsa stain; cell cycle phase distribution and hypodiploid DNA were determined by flow cytometry with PI; expressions of Livin, Caspase-3 were detected by Western blot.
Results: MTT assay showed that Bufalin inhibited A549 cell proliferation in time and dose-dependent manner. The inhibition concentration of 50% cell growth (IC50) at 48 h, 72 h and 96 h were (56.14+/-6.72) nmol/L, (15.57+/-4.28) nmol/L and (7.39+/-4.16) nmol/L, respectively; apoptosis of A549 cells could be effectively induced by Bufalin; apoptotic bodies and sub-G1 peak (representing apoptosis) emerged when cells were treated with different concentrations, compared with the untreated control group (P < 0.01). The expression of Livin protein was downregulated (P < 0.01) and Caspase-3 protein was activated during Bufalin-induced apoptosis in A549 cells.
Conclusions: Bufalin suppresses the growth of A549 cells and induces apoptosis; inhibition of Livin and activation of Caspase-3 may be involved in its mechanism.

Retraction in: Zhongguo Fei Ai Za Zhi. 2010 Nov;13(11):1045. (PMID: 21081045)

Informacja