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Tytuł:
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[Effects of 5-bromotetrandrine and daunorubicin on apoptosis and expression of survivin in K562/A02 cells].
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Autorzy:
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Cai XH; Department of Hematology, Medical School of Southeast University, Nanjing 210009, Jiangsu Province, China.
Chen BA
Cheng J
Wang J
Ding JH
Bao W
Zhong YJ
Gao F
Xu WL
Shen HL
Wei HL
Chen J
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Źródło:
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Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2011 Feb; Vol. 19 (1), pp. 24-7.
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Typ publikacji:
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Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
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Język:
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Chinese
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Imprint Name(s):
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Original Publication: Beijing : Zhongguo shi yan xue za zhi she,
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MeSH Terms:
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Apoptosis/*drug effects
Benzylisoquinolines/*pharmacology
Daunorubicin/*pharmacology
Inhibitor of Apoptosis Proteins/*genetics
Apoptosis/genetics ; Drug Resistance, Multiple/genetics ; Drug Resistance, Neoplasm/genetics ; Gene Expression Regulation, Leukemic ; Humans ; K562 Cells ; Survivin
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Substance Nomenclature:
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0 (BIRC5 protein, human)
0 (Benzylisoquinolines)
0 (Inhibitor of Apoptosis Proteins)
0 (Survivin)
0 (bromotetrandrine)
ZS7284E0ZP (Daunorubicin)
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Entry Date(s):
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Date Created: 20110303 Date Completed: 20121217 Latest Revision: 20181201
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Update Code:
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20240104
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PMID:
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21362215
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The aim of this study was to investigate the potential benefit of combination therapy with 5-bromotetrandrine (5-BrTet) and daunorubicin (DNR) on chronic leukemia. The apoptosis of K562/A02 cells treated by DNA, BrTet and BrTet combined with DNR for 48 hours was detected by flow cytometry; the expressions levels of survivin mRNA and protein K562/A02 cells treated by DNR, BrTet and BrTet combined with DNR and in untreated K562 cells for 48 hours were measured by RT-PCR and Western blot respectively. The results showed that the combination of BrTet with DNR increased apoptotic rate of K562/A02, down-regulated the expression levels of survivin mRNA and protein in K562/A02 cells as compared with blank control and cells treated by BrTet or DNR alone, the survivin expression in K562/A02 cells was higher than that in K562 cells. It is concluded that the combination of BrTet with DNR can effectively reverse the multidrug resistance of K562/A02 cells, promote the apoptosis of K562/A02 cells, the mechanism of which may be related with down-regulation of survivin expression. Survivin may be a target for the treatment of MDR in hematopoietic malignancies.
