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Tytuł pozycji:

Quantification of both the presence, and oxidation state, of Mn in Bacillus atrophaeus spores and its imparting of magnetic susceptibility to the spores.

Tytuł:
Quantification of both the presence, and oxidation state, of Mn in Bacillus atrophaeus spores and its imparting of magnetic susceptibility to the spores.
Autorzy:
Sun J; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
Zborowski M
Chalmers JJ
Źródło:
Biotechnology and bioengineering [Biotechnol Bioeng] 2011 May; Vol. 108 (5), pp. 1119-29. Date of Electronic Publication: 2011 Jan 04.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
Język:
English
Imprint Name(s):
Publication: <2005->: Hoboken, NJ : Wiley
Original Publication: New York, Wiley.
MeSH Terms:
Magnetics*
Bacillus/*physiology
Spores, Bacterial/*growth & development
Mass Spectrometry/methods ; Oxidation-Reduction
References:
Acc Chem Res. 2010 Jan 19;43(1):2-9. (PMID: 19778036)
Int J Food Microbiol. 2005 Jun 25;102(1):107-11. (PMID: 15925006)
Analyst. 2005 Apr;130(4):514-27. (PMID: 15776162)
Anal Chem. 2005 Feb 15;77(4):1081-7. (PMID: 15858989)
Rapid Commun Mass Spectrom. 1999;13(15):1580-5. (PMID: 10421900)
J Physiol Biochem. 2009 Jun;65(2):195-208. (PMID: 19886398)
Appl Opt. 1997 Feb 1;36(4):958-67. (PMID: 18250761)
Langmuir. 2005 Nov 8;21(23):10710-6. (PMID: 16262341)
J Gen Microbiol. 1989 Oct;135(10):2717-22. (PMID: 2517297)
Cytometry. 1998 Dec 1;33(4):469-75. (PMID: 9845442)
Biotechnol Bioeng. 2010 Apr 15;105(6):1078-93. (PMID: 20014141)
J Microbiol Methods. 2006 Oct;67(1):56-63. (PMID: 16616384)
Microbes Infect. 2000 Apr;2(4):391-400. (PMID: 10817641)
Int J Syst Evol Microbiol. 2001 Jan;51(Pt 1):35-37. (PMID: 11211269)
J Microbiol Methods. 2000 May;40(3):241-54. (PMID: 10802141)
J Appl Microbiol. 2005;98(2):351-63. (PMID: 15659190)
Pharmacol Res. 2006 Nov;54(5):389-95. (PMID: 17000117)
Appl Microbiol. 1972 Sep;24(3):497-8. (PMID: 4627969)
Biotechnol Bioeng. 2007 Sep 1;98(1):186-92. (PMID: 17335063)
J Appl Microbiol. 2008 Jul;105(1):68-77. (PMID: 18298528)
Analyst. 2008 Dec;133(12):1767-75. (PMID: 19082082)
Appl Environ Microbiol. 1988 Aug;54(8):2140-3. (PMID: 16347723)
Appl Environ Microbiol. 2004 May;70(5):2786-90. (PMID: 15128533)
Grant Information:
R01 CA062349 United States CA NCI NIH HHS; R01 CA097391 United States CA NCI NIH HHS; UL1 RR025755 United States RR NCRR NIH HHS; R01 CA62349 United States CA NCI NIH HHS
Entry Date(s):
Date Created: 20110331 Date Completed: 20110712 Latest Revision: 20211020
Update Code:
20240104
PubMed Central ID:
PMC3431158
DOI:
10.1002/bit.23034
PMID:
21449026
Czasopismo naukowe
Bacillus atrophaeus spores were previously reported to have significant magnetic susceptibility in a magnetic field due to the presence of Mn. However, relatively little is known about the total amount and distribution of the oxidation state of Mn associated with this specific strain's spores. Using the instrument, cell tracking velocimetry (CTV) both magnetically induced velocity and settling velocity was quantitatively measured. Visual observations, and calculated diameter using previously reported densities, indicate that the spores are present in the form of clusters of approximately 3-6 µm. Treatment of these clusters with EDTA or pH of 2.0 or below resulted in not only the disruption of the spore clusters, but also a significant decrease in magnetic susceptibility, in some cases by almost two orders of magnitude. Since the magnetic susceptibility of Mn varies significantly between the three typically reported valance states of Mn, Mn(II), Mn(III), and Mn(IV); X-Ray Photoelectron Spectroscopy, XPS, was used to determined the valance states of Mn in the spores. This XPS analysis, which penetrates up to 10 nm into the spore, returned the following fractions: 0.41, 0.38, and 0.21 for the valance states: Mn(II), Mn(III), and Mn(IV), respectively. The total mass of Mn associated with each spore cluster was determined by ICP-MS. A second, completely independent estimate of Mn mass associated with each spore cluster was made, by mathematically solving for the amount of Mn per spore cluster using the experimentally measured magnetophoretic mobility and the magnetic susceptibility of each of the three valence states from the XPS analysis. IPC-MS returned a value of 3.28 × 10(-11) g of Mn per spore cluster while the calculated estimation from mobility and XPS analysis retuned a value of 1.16 × 10(-11) g, which given the complexity of the two techniques, is a reasonable agreement. Finally, a discussion of potential applications of the magnetic properties of these spores is presented.
(Copyright © 2010 Wiley Periodicals, Inc.)

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