Casuarinin suppresses TNF-α-induced ICAM-1 expression via blockade of NF-κB activation in HaCaT cells.

Szczegóły
Abstrakt

Tytuł:: Casuarinin suppresses TNF-α-induced ICAM-1 expression via blockade of NF-κB activation in HaCaT cells.
Autorzy:: Kwon DJ; Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea.
Bae YS
Ju SM
Goh AR
Choi SY
Park J
Źródło:: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Jun 17; Vol. 409 (4), pp. 780-5. Date of Electronic Publication: 2011 May 20.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
MeSH Terms:: Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
Hydrolyzable Tannins/*pharmacology
Intercellular Adhesion Molecule-1/*biosynthesis
Keratinocytes/*drug effects
NF-kappa B/*antagonists & inhibitors
Anti-Inflammatory Agents, Non-Steroidal/chemistry ; Cell Line ; Chemokine CCL2/antagonists & inhibitors ; Dermatitis/drug therapy ; Dermatitis/immunology ; Hippophae/chemistry ; Humans ; Hydrolyzable Tannins/isolation & purification ; Interleukin-1beta/antagonists & inhibitors ; Interleukin-6/antagonists & inhibitors ; Interleukin-8/antagonists & inhibitors ; Keratinocytes/immunology ; Plant Leaves/chemistry ; Tumor Necrosis Factor-alpha/pharmacology
Substance Nomenclature:: 0 (Anti-Inflammatory Agents, Non-Steroidal)
0 (Chemokine CCL2)
0 (Hydrolyzable Tannins)
0 (Interleukin-1beta)
0 (Interleukin-6)
0 (Interleukin-8)
0 (NF-kappa B)
0 (Tumor Necrosis Factor-alpha)
0 (casuarinin)
126547-89-5 (Intercellular Adhesion Molecule-1)
Entry Date(s):: Date Created: 20110531 Date Completed: 20110902 Latest Revision: 20220310
Update Code:: 20240104
DOI:: 10.1016/j.bbrc.2011.05.088
PMID:: 21621513
: Czasopismo naukowe

Full Text Finder

Hippophae rhamnoides has been extensively used in oriental traditional medicines for treatment of asthma, skin diseases, gastric ulcers, and lung disorders. In this study, we isolated casuarinin from the leaves of H.rhamnoides and examined the effect of casuarinin on the TNF-α-induced ICAM-1 expression in a human keratinocytes cell line HaCaT. Pretreatment with casuarinin inhibited TNF-α-induced protein and mRNA expression of ICAM-1 and subsequent monocyte adhesiveness in HaCaT cells. Casuarinin significantly inhibited TNF-α-induced NF-κB activation. In addition, casuarinin inhibited activation of ERK and p38 MAPK in a dose-dependent manner. Furthermore, pretreatment with casuarinin decreased TNF-α-induced pro-inflammatory mediators, such as IL-1β, IL-6, IL-8, and MCP-1. These results demonstrated that casuarinin exerts its anti-inflammatory activity by suppressing TNF-α-induced expression of ICAM-1 and pro-inflammatory cytokines/chemokines via blockage of activation of NF-κB and ERK/p38 MAPK and can be used as a therapeutic agent against inflammatory skin diseases.
(Copyright © 2011 Elsevier Inc. All rights reserved.)

Informacja