Tytuł pozycji:
A water soluble vitamin B12-ReI fluorescent conjugate for cell uptake screens: use in the confirmation of cubilin in the lung cancer line A549.
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Tytuł:
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A water soluble vitamin B12-ReI fluorescent conjugate for cell uptake screens: use in the confirmation of cubilin in the lung cancer line A549.
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Autorzy:
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Vortherms AR; Department of Chemistry, Centre for Science and Technology, Syracuse University, Syracuse, NY 13244, USA.
Kahkoska AR
Rabideau AE
Zubieta J
Andersen LL
Madsen M
Doyle RP
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Źródło:
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Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2011 Sep 21; Vol. 47 (35), pp. 9792-4. Date of Electronic Publication: 2011 Aug 05.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Cambridge : Royal Society of Chemistry
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MeSH Terms:
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Lung Neoplasms/*pathology
Receptors, Cell Surface/*genetics
Rhenium/*chemistry
Vitamin B 12/*chemistry
Vitamin B 12/*metabolism
Water/*chemistry
Biological Transport ; Cell Line, Tumor ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Lung Neoplasms/genetics ; Solubility
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Substance Nomenclature:
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0 (Fluorescent Dyes)
0 (Receptors, Cell Surface)
0 (intrinsic factor-cobalamin receptor)
059QF0KO0R (Water)
7440-15-5 (Rhenium)
P6YC3EG204 (Vitamin B 12)
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Entry Date(s):
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Date Created: 20110806 Date Completed: 20111219 Latest Revision: 20131121
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Update Code:
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20240104
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DOI:
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10.1039/c1cc13615a
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PMID:
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21818500
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A water soluble vitamin B(12)-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.