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Tytuł:
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Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration.
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Autorzy:
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Gaebel R; Reference- and Translation Center for Cardiac Stem Cell Therapy, Department of Cardiac Surgery, University of Rostock, 18057 Rostock, Germany.
Ma N
Liu J
Guan J
Koch L
Klopsch C
Gruene M
Toelk A
Wang W
Mark P
Wang F
Chichkov B
Li W
Steinhoff G
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Źródło:
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Biomaterials [Biomaterials] 2011 Dec; Vol. 32 (35), pp. 9218-30. Date of Electronic Publication: 2011 Sep 10.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: <1995-> : Amsterdam : Elsevier Science
Original Publication: [Guilford, England] : IPC Science and Technology Press, 1980-
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MeSH Terms:
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Lasers*
Heart/*physiology
Human Umbilical Vein Endothelial Cells/*cytology
Mesenchymal Stem Cells/*cytology
Regeneration/*physiology
Regenerative Medicine/*methods
Animals ; Capillaries/drug effects ; Capillaries/pathology ; Cell Movement/drug effects ; Cell Separation ; Cells, Cultured ; Fibrosis ; Heart/drug effects ; Heart Function Tests/drug effects ; Human Umbilical Vein Endothelial Cells/drug effects ; Human Umbilical Vein Endothelial Cells/metabolism ; Humans ; Immunophenotyping ; Implants, Experimental ; Mesenchymal Stem Cells/drug effects ; Mesenchymal Stem Cells/metabolism ; Myocardial Infarction/pathology ; Myocardial Infarction/physiopathology ; Neovascularization, Physiologic/drug effects ; Rats ; Regeneration/drug effects ; Tissue Scaffolds/chemistry ; Urethane/pharmacology
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Substance Nomenclature:
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3IN71E75Z5 (Urethane)
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Entry Date(s):
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Date Created: 20110914 Date Completed: 20120203 Latest Revision: 20220316
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Update Code:
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20240104
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DOI:
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10.1016/j.biomaterials.2011.08.071
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PMID:
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21911255
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Recent study showed that mesenchymal stem cells (MSC) could inhibit apoptosis of endothelial cells in hypoxic condition, increase their survival, and stimulate the angiogenesis process. In this project we applied Laser-Induced-Forward-Transfer (LIFT) cell printing technique and prepared a cardiac patch seeded with human umbilical vein endothelial cells (HUVEC) and human MSC (hMSC) in a defined pattern for cardiac regeneration. We seeded HUVEC and hMSC in a defined pattern on a Polyester urethane urea (PEUU) cardiac patch. On control patches an equal amount of cells was randomly seeded without LIFT. Patches were cultivated in vitro or transplanted in vivo to the infarcted zone of rat hearts after LAD-ligation. Cardiac performance was measured by left ventricular catheterization 8 weeks post infarction. Thereafter hearts were perfused with fluorescein tomato lectin for the assessment of functional blood vessels and stored for histology analyses. We demonstrated that LIFT-derived cell seeding pattern definitely modified growth characteristics of co-cultured HUVEC and hMSC leading to increased vessel formation and found significant functional improvement of infarcted hearts following transplantation of a LIFT-tissue engineered cardiac patch. Further, we could show enhanced capillary density and integration of human cells into the functionally connected vessels of murine vascular system. LIFT-based Tissue Engineering of cardiac patches for the treatment of myocardial infarction might improve wound healing and functional preservation.
(Copyright © 2011. Published by Elsevier Ltd.)
