Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Metabolic fingerprinting using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry.

Tytuł:
Metabolic fingerprinting using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry.
Autorzy:
Almstetter MF; Institute of Functional Genomics, University of Regensburg, Josef-Engert-Str. 9, Regensburg 93053, Germany.
Oefner PJ
Dettmer K
Źródło:
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 815, pp. 399-411.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:
Gas Chromatography-Mass Spectrometry*/standards
Blood Chemical Analysis/*methods
Blood Chemical Analysis/standards ; Cluster Analysis ; Fatty Acids/standards ; Humans ; Metabolomics ; Multivariate Analysis ; Principal Component Analysis
Substance Nomenclature:
0 (Fatty Acids)
Entry Date(s):
Date Created: 20111202 Date Completed: 20120320 Latest Revision: 20111201
Update Code:
20240104
DOI:
10.1007/978-1-61779-424-7_29
PMID:
22131007
Czasopismo naukowe
Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF-MS) is applied to the comparative metabolic fingerprinting of physiological fluids. Stable isotope-labeled internal standards plus norvaline serve as extraction standards and are added to the blanks, controls and patient samples prior to protein precipitation with methanol. The extracts are evaporated to complete dryness and derivatized in two steps using methoximation with methoxylamine hydrochloride (MeOx) and silylation with N-methyl-N-trimethylsily-trifluoroacetamide (MSTFA). Between derivatization steps a second internal standard containing odd-numbered, saturated straight chain fatty acids is added for quality control and to normalize retention time shifts. After GC × GC-TOF-MS analysis raw data are processed, aligned, and combined in one data matrix for subsequent statistical evaluation. Both a custom-made and the NIST 05 library are used to preliminarily identify significant metabolites. For verification purposes, commercial standards are run individually. Absolute quantification of selected metabolites is achieved by using a multi-point calibration curve and isotope-labeled internal standards.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies