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Tytuł pozycji:

Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage.

Tytuł:
Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage.
Autorzy:
Mak SK; Basic Research Department, The Parkinson's Institute, 675 Almanor Ave, Sunnyvale, CA 94085, USA.
Huang YA
Iranmanesh S
Vangipuram M
Sundararajan R
Nguyen L
Langston JW
Schüle B
Źródło:
Stem cells international [Stem Cells Int] 2012; Vol. 2012, pp. 140427. Date of Electronic Publication: 2012 Apr 10.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: 2011- : New York : Hindawi
Original Publication: London, England ; New York, NY : SAGE-Hindawi Access to Research
References:
Nat Biotechnol. 2009 Mar;27(3):275-80. (PMID: 19252484)
Cell. 2011 Jul 8;146(1):37-52. (PMID: 21700325)
Circulation. 2001 Sep 18;104(12):E9027-8. (PMID: 11565575)
Cell Stem Cell. 2011 Jan 7;8(1):31-45. (PMID: 21185252)
Nat Protoc. 2008;3(7):1180-6. (PMID: 18600223)
Stem Cells Dev. 2011 Oct;20(10):1733-46. (PMID: 21226567)
Trends Endocrinol Metab. 2008 Oct;19(8):300-7. (PMID: 18771935)
Neuron. 2000 Oct;28(1):31-40. (PMID: 11086981)
Nat Biotechnol. 2000 Jun;18(6):675-9. (PMID: 10835609)
Mol Pharmacol. 2002 Jul;62(1):65-74. (PMID: 12065756)
PLoS One. 2009 Jul 14;4(7):e6233. (PMID: 19597550)
J Microsc. 1987 Sep;147(Pt 3):229-63. (PMID: 3430576)
Neurosci Res. 2010 Nov;68(3):176-84. (PMID: 20708045)
Stem Cells. 2008 Jun;26(6):1517-25. (PMID: 18388303)
Nature. 2009 Jan 15;457(7227):277-80. (PMID: 19098894)
Nat Chem Biol. 2008 Jan;4(1):33-41. (PMID: 18026094)
Cell Stem Cell. 2011 Jun 3;8(6):695-706. (PMID: 21624813)
Anat Rec. 1991 Dec;231(4):482-97. (PMID: 1793176)
Cell Stem Cell. 2010 May 7;6(5):407-11. (PMID: 20452313)
Mol Cell Neurosci. 2011 May;47(1):36-44. (PMID: 21362477)
Genes Cells. 2011 Jul;16(7):778-90. (PMID: 21668588)
Cell Transplant. 2012;21(1):313-32. (PMID: 21669041)
Stem Cell Rev Rep. 2010 Jun;6(2):270-81. (PMID: 20376579)
Curr Opin Neurol. 2010 Aug;23(4):426-32. (PMID: 20489615)
Cell Stem Cell. 2011 Mar 4;8(3):267-80. (PMID: 21362567)
Stem Cells. 2004;22(6):925-40. (PMID: 15536184)
Nat Commun. 2011 Aug 23;2:440. (PMID: 21863007)
J Neurosci Res. 2011 Feb;89(2):117-26. (PMID: 21162120)
J Neurosci. 2011 Apr 20;31(16):5970-6. (PMID: 21508222)
Nat Biotechnol. 2001 Dec;19(12):1129-33. (PMID: 11731781)
Nature. 2011 Nov 06;480(7378):547-51. (PMID: 22056989)
Biochim Biophys Acta. 2009 Nov;1792(11):1043-51. (PMID: 19733239)
Neurosci Lett. 2011 Jan 13;488(1):36-40. (PMID: 21056624)
Semin Cell Dev Biol. 2003 Jun;14(3):181-9. (PMID: 12948353)
Neuroreport. 2003 Aug 6;14(11):1467-70. (PMID: 12960765)
Exp Cell Res. 2010 Apr 15;316(7):1148-58. (PMID: 20171210)
Nat Rev Drug Discov. 2010 May;9(5):367-72. (PMID: 20339370)
Stem Cells. 2010 Oct;28(10):1893-904. (PMID: 20715183)
Methods Mol Biol. 2011;793:87-97. (PMID: 21913095)
J Neuroendocrinol. 2009 Aug;21(8):730-6. (PMID: 19500215)
Cell. 2007 Nov 30;131(5):861-72. (PMID: 18035408)
Int J Dev Neurosci. 2009 Aug;27(5):493-500. (PMID: 19379802)
Am Fam Physician. 2002 Mar 15;65(6):1155-8, 1161-2, 1164. (PMID: 11925094)
Stem Cells. 2010 Aug;28(8):1326-37. (PMID: 20641036)
Stem Cells. 2006 Jun;24(6):1583-93. (PMID: 16543488)
Stem Cell Rev. 2005;1(2):83-5. (PMID: 17142842)
Adv Exp Med Biol. 2009;651:101-11. (PMID: 19731555)
Entry Date(s):
Date Created: 20120509 Date Completed: 20120823 Latest Revision: 20211021
Update Code:
20240104
PubMed Central ID:
PMC3339118
DOI:
10.1155/2012/140427
PMID:
22567022
Czasopismo naukowe
Efficient in vitro differentiation into specific cell types is more important than ever after the breakthrough in nuclear reprogramming of somatic cells and its potential for disease modeling and drug screening. Key success factors for neuronal differentiation are the yield of desired neuronal marker expression, reproducibility, length, and cost. Three main neuronal differentiation approaches are stromal-induced neuronal differentiation, embryoid body (EB) differentiation, and direct neuronal differentiation. Here, we describe our neurodifferentiation protocol using small molecules that very efficiently promote neural induction in a 5-stage EB protocol from six induced pluripotent stem cells (iPSC) lines from patients with Parkinson's disease and controls. This protocol generates neural precursors using Dorsomorphin and SB431542 and further maturation into dopaminergic neurons by replacing sonic hedgehog with purmorphamine or smoothened agonist. The advantage of this approach is that all patient-specific iPSC lines tested in this study were successfully and consistently coaxed into the neural lineage.

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