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Tytuł:
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Protein thioester synthesis enabled by sortase.
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Autorzy:
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Ling JJ; Department of Chemistry, Massachusetts Institute of Technology, 16-573a, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Policarpo RL
Rabideau AE
Liao X
Pentelute BL
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Źródło:
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Journal of the American Chemical Society [J Am Chem Soc] 2012 Jul 04; Vol. 134 (26), pp. 10749-52. Date of Electronic Publication: 2012 Jun 19.
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Typ publikacji:
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Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
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Język:
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English
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Imprint Name(s):
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Publication: Washington, DC : American Chemical Society
Original Publication: Easton, Pa. [etc.]
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MeSH Terms:
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Aminoacyltransferases/*metabolism
Bacterial Proteins/*metabolism
Cysteine Endopeptidases/*metabolism
Esters/*chemistry
Proteins/*chemical synthesis
Sulfhydryl Compounds/*chemistry
Antigens, Bacterial/chemistry ; Bacterial Toxins/chemistry ; Inteins ; Peptides/chemical synthesis ; Protein Transport ; Recombinant Proteins/chemistry ; Sulfur Compounds
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References:
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Grant Information:
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U54 AI057159 United States AI NIAID NIH HHS; AI057159 United States AI NIAID NIH HHS
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Substance Nomenclature:
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0 (Antigens, Bacterial)
0 (Bacterial Proteins)
0 (Bacterial Toxins)
0 (Esters)
0 (Peptides)
0 (Proteins)
0 (Recombinant Proteins)
0 (Sulfhydryl Compounds)
0 (Sulfur Compounds)
0 (anthrax toxin)
EC 2.3.2.- (Aminoacyltransferases)
EC 2.3.2.- (sortase A)
EC 3.4.22.- (Cysteine Endopeptidases)
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Entry Date(s):
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Date Created: 20120613 Date Completed: 20121011 Latest Revision: 20211021
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Update Code:
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20240104
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PubMed Central ID:
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PMC3465687
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DOI:
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10.1021/ja302354v
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PMID:
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22686546
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Proteins containing a C-terminal thioester are important intermediates in semisynthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. Here we report a simple strategy, utilizing sortase A, for routine preparation of recombinant proteins containing a C-terminal (α)thioester. We used our method to prepare two different anthrax toxin cargo proteins: one containing an (α)thioester and another containing a D-polypeptide segment situated between two protein domains. We show that both variants can translocate through protective antigen pore. This new method to synthesize a protein thioester allows for interfacing of sortase-mediated ligation and native chemical ligation.