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Tytuł:
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Low-level laser therapy and sodium diclofenac in acute inflammatory response induced by skeletal muscle trauma: effects in muscle morphology and mRNA gene expression of inflammatory markers.
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Autorzy:
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de Almeida P; Post Graduate Program in Rehabilitation Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
Lopes-Martins RÁ
Tomazoni SS
Albuquerque-Pontes GM
Santos LA
Vanin AA
Frigo L
Vieira RP
Albertini R
de Carvalho Pde T
Leal-Junior EC
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Źródło:
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Photochemistry and photobiology [Photochem Photobiol] 2013 Mar-Apr; Vol. 89 (2), pp. 501-7. Date of Electronic Publication: 2012 Oct 09.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: <2004->: Lawrence KS : American Society for Photobiology
Original Publication: Augusta, GA: American Society for Photobiology, <1996->
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MeSH Terms:
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Low-Level Light Therapy*
Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
Diclofenac/*pharmacology
Muscle, Skeletal/*radiation effects
Soft Tissue Injuries/*radiotherapy
Animals ; Biomarkers/analysis ; Cyclooxygenase 1/genetics ; Cyclooxygenase 1/immunology ; Cyclooxygenase 2/genetics ; Cyclooxygenase 2/immunology ; Gene Expression/radiation effects ; Inflammation/prevention & control ; Male ; Membrane Proteins/genetics ; Membrane Proteins/immunology ; Muscle, Skeletal/drug effects ; Muscle, Skeletal/injuries ; Muscle, Skeletal/metabolism ; Rats ; Rats, Wistar ; Soft Tissue Injuries/drug therapy ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/immunology
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Substance Nomenclature:
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0 (Anti-Inflammatory Agents, Non-Steroidal)
0 (Biomarkers)
0 (Membrane Proteins)
0 (Tumor Necrosis Factor-alpha)
144O8QL0L1 (Diclofenac)
EC 1.14.99.1 (Cyclooxygenase 1)
EC 1.14.99.1 (Cyclooxygenase 2)
EC 1.14.99.1 (Ptgs1 protein, rat)
EC 1.14.99.1 (Ptgs2 protein, rat)
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Entry Date(s):
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Date Created: 20120904 Date Completed: 20131115 Latest Revision: 20220408
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Update Code:
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20240104
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DOI:
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10.1111/j.1751-1097.2012.01232.x
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PMID:
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22937980
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Pharmacological therapy is widely used in the treatment of muscle injuries. On the other hand, low-level laser therapy (LLLT) arises as a promising nonpharmacological treatment. The aim of this study was to analyze the effects of sodium diclofenac (topical application) and LLLT on morphological aspects and gene expression of biochemical inflammatory markers. We performed a single trauma in tibialis anterior muscle of rats. After 1 h, animals were treated with sodium diclofenac (11.6 mg g(-1) of solution) or LLLT (810 nm; continuous mode; 100 mW; 3.57 W cm(-2) ; 1, 3 or 9 J; 10, 30 or 90 s). Histological analysis and quantification of gene expression (real-time polymerase chain reaction-RT-PCR) of cyclooxygenase 1 and 2 (COX-1 and COX-2) and tumor necrosis factor-alpha (TNF-α) were performed at 6, 12 and 24 h after trauma. LLLT with all doses improved morphological aspects of muscle tissue, showing better results than injury and diclofenac groups. All LLLT doses also decreased (P < 0.05) COX-2 compared to injury group at all time points, and to diclofenac group at 24 h after trauma. In addition, LLLT decreased (P < 0.05) TNF-α compared both to injury and diclofenac groups at all time points. LLLT mainly with dose of 9 J is better than topical application of diclofenac in acute inflammation after muscle trauma.
(© 2012 CSIR Photochemistry and Photobiology © 2012 The American Society of Photobiology.)