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Tytuł pozycji:

The antioxidant activity of sulphurous thermal water protects against oxidative DNA damage: a comet assay investigation.

Tytuł:
The antioxidant activity of sulphurous thermal water protects against oxidative DNA damage: a comet assay investigation.
Autorzy:
Braga PC; Department of Medical Biotechnology and Translational Medicine, School of Medicine, Centre of Respiratory Pharmacology, University of Milan, Milan, Italy. />Ceci C
Marabini L
Nappi G
Źródło:
Drug research [Drug Res (Stuttg)] 2013 Apr; Vol. 63 (4), pp. 198-202. Date of Electronic Publication: 2013 Feb 27.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Stuttgart : Thieme
MeSH Terms:
DNA Damage*
Antioxidants/*pharmacology
Comet Assay/*methods
Sulfur/*pharmacology
Cell Line, Tumor ; Humans ; Hydrogen-Ion Concentration ; Oxidation-Reduction ; Temperature ; Water/pharmacology
Substance Nomenclature:
0 (Antioxidants)
059QF0KO0R (Water)
70FD1KFU70 (Sulfur)
Entry Date(s):
Date Created: 20130301 Date Completed: 20130620 Latest Revision: 20131213
Update Code:
20240104
DOI:
10.1055/s-0033-1334894
PMID:
23447143
Czasopismo naukowe
Various studies have recently shown that sulphurous waters acts against the oxidants released during respiratory bursts of human neutrophils, and free radicals such as HO•, O2¯•, Tempol and Fremy's salt. However, there is still a lack of data concerning their direct protection of DNA. The aim of this study was to investigate the antigenotoxicity effects of sulphurous water, which has never been previously investigated for this purpose, using the alkaline single cell gel electrophoresis (SCGE) approach (comet assay). The comet assay is a sensitive method for assessing DNA fragmentation in individual cells in genotoxicity studies but can also be used to investigate the activity of agents that protect against DNA damage. The extent of migration was measured by means of SCGE, and DNA damage was expressed as tail moment. All of these assays were made using natural sulphurous water, degassed sulphurous water (no detectable HS), and reconstituted sulphurous water (degassed plus NaHS). DNA damages was significantly inhibited by natural water with HS concentrations of 5.0 and 2.5 μg/mL. The use of degassed water did not lead to any significant differences from baseline values, whereas the reconstituted water led to significant results overlapping those obtained using natural water. These findings confirm the importance of the presence of an HS group (reductive activity) and indicate that, in addition to their known mucolytic activity and trophic effects on respiratory mucosa, HS groups in sulphurous water also protect against oxidative DNA damage and contribute to the water's therapeutic effects on upper and lower airway inflammatory diseases.
(© Georg Thieme Verlag KG Stuttgart · New York.)

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