Schistosoma japonicum: Tsunagi/Y14 protein plays a critical role in the development of the reproductive organs and eggs.

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Abstrakt

Tytuł:: Schistosoma japonicum: Tsunagi/Y14 protein plays a critical role in the development of the reproductive organs and eggs.
Autorzy:: Ren CP; Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Medical University, 81# Meishan Road, Hefei, Anhui 230032, People's Republic of China.
Zhang P
Zhang WN
Huang DK
Jia XM
Gui L
Liu M
Shen JJ
Źródło:: Experimental parasitology [Exp Parasitol] 2013 Oct; Vol. 135 (2), pp. 430-6. Date of Electronic Publication: 2013 Aug 23.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: Orlando, FL : Academic Press
Original Publication: New York.
MeSH Terms:: Drosophila Proteins/*physiology
RNA-Binding Proteins/*physiology
Schistosoma japonicum/*physiology
Animals ; Drosophila Proteins/genetics ; Female ; Liver/parasitology ; Liver/pathology ; Male ; Mice ; Mice, Inbred BALB C ; RNA Interference ; RNA, Double-Stranded/genetics ; RNA, Double-Stranded/metabolism ; RNA, Helminth/genetics ; RNA, Helminth/metabolism ; RNA-Binding Proteins/genetics ; Real-Time Polymerase Chain Reaction ; Schistosoma japonicum/growth & development ; Schistosomiasis japonica/parasitology ; Schistosomiasis japonica/pathology ; Snails ; Specific Pathogen-Free Organisms
Contributed Indexing:: Keywords: RNAi; Reproduction; Reproductive organs; Schistosoma japonicum; Tsunagi/Y14; dsRNA
Substance Nomenclature:: 0 (Drosophila Proteins)
0 (RNA, Double-Stranded)
0 (RNA, Helminth)
0 (RNA-Binding Proteins)
0 (tsu protein, Drosophila)
Entry Date(s):: Date Created: 20130827 Date Completed: 20131205 Latest Revision: 20190607
Update Code:: 20240104
DOI:: 10.1016/j.exppara.2013.08.001
PMID:: 23973739
: Czasopismo naukowe

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Tsunagi/Y14 is an evolutionarily conserved RNA-binding protein that is required for the maintenance of oogenesis and the masculinization of the germ-line in many animal models. We speculated that Tsunagi/Y14 might also regulate reproductive organ development in Schistosoma japonicum (S. japonicum, Sj). Sj Tsunagi/Y14 and control double-stranded RNAs were introduced into schistosomula by electroporation respectively. These transfected schistosomula were cultured in vitro for 1, 3 or 5 days. The mRNA and protein levels of the target gene in the cultured schistosomula were significantly suppressed compared with those of the control group. Furthermore, BALB/c mice were infected with the transfected schistosomula for 6 weeks and were sacrificed to harvest the adult worms. We found that the silencing of Sj Tsunagi/Y14 led to defects in reproductive organs development in both male and female worms. Moreover, it also affected the size, quantity and activity of the eggs in the mice liver. Our findings indicated that Tsunagi/Y14 plays a critical role in the development of reproductive organs and eggs in S. japonicum.
(Copyright © 2013 Elsevier Inc. All rights reserved.)

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