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Tytuł pozycji:

Protective effect of Vaccinium myrtillus extract against UVA- and UVB-induced damage in a human keratinocyte cell line (HaCaT cells).

Tytuł :
Protective effect of Vaccinium myrtillus extract against UVA- and UVB-induced damage in a human keratinocyte cell line (HaCaT cells).
Autorzy :
Calò R; Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy. Electronic address: .
Marabini L; Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy.
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Źródło :
Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2014 Mar 05; Vol. 132, pp. 27-35. Date of Electronic Publication: 2014 Feb 08.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Original Publication: Lausanne : Elsevier Sequoia, 1987-
MeSH Terms :
Ultraviolet Rays*
DNA Damage/*radiation effects
Keratinocytes/*drug effects
Plant Extracts/*pharmacology
Protective Agents/*pharmacology
Vaccinium/*chemistry
Cell Line ; Cell Survival/drug effects ; Cell Survival/radiation effects ; Comet Assay ; Humans ; Keratinocytes/cytology ; Keratinocytes/radiation effects ; Lipid Peroxidation/drug effects ; Lipid Peroxidation/radiation effects ; Membrane Potential, Mitochondrial/drug effects ; Membrane Potential, Mitochondrial/radiation effects ; Micronucleus Tests ; Oxidative Stress/drug effects ; Oxidative Stress/radiation effects ; Plant Extracts/chemistry ; Protective Agents/chemistry ; Reactive Oxygen Species/metabolism ; Vaccinium/metabolism
Contributed Indexing :
Keywords: Apoptosis; Genotoxicity; Keratinocytes; Oxidative stress; Ultraviolet rays; Vaccinium myrtillus
Substance Nomenclature :
0 (Plant Extracts)
0 (Protective Agents)
0 (Reactive Oxygen Species)
Entry Date(s) :
Date Created: 20140301 Date Completed: 20141203 Latest Revision: 20140325
Update Code :
20211105
DOI :
10.1016/j.jphotobiol.2014.01.013
PMID :
24577051
Czasopismo naukowe
Recently, the field of skin protection have shown a considerable interest in the use of botanicals. Vaccinium myrtillus contains several polyphenols and anthocyanins with multiple pharmacological properties. The purpose of our study was to examine whether a water-soluble V. myrtillus extract (dry matter 12.4%; total polyphenols 339.3mg/100 g fw; total anthocyanins 297.4 mg/100 g fw) was able to reduce UVA- and UVB-induced damage using a human keratinocyte cell line (HaCaT). HaCaT cells were pretreated for 1h with extract in a serum-free medium and then irradiated with UVA (8-40 J/cm(2)) and UVB (0.008-0.72 J/cm(2)) rays. All experiments were performed 24h after the end of irradiation, except for oxidative stress tests. The extract was able to reduce the UVB-induced cytotoxicity and genotoxicity (studied by comet and micronucleous assays) at lower doses. V. myrtillus extract reduced lipid peroxidation UVB-induced, but had no effect against the ROS UVB-produced. With UVA-induced damage V. myrtillus reduced genotoxicity as well as the unbalance of redox intracellular status. Moreover our extract reduced the UVA-induced apoptosis, but had no effect against the UVB one. V. myrtillus extract showed its free radical scavenging properties reducing oxidative stress and apoptotic markers, especially in UVA-irradiated cells.
(Copyright © 2014 Elsevier B.V. All rights reserved.)

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