Microbial based assay for specific detection of β-lactam group of antibiotics in milk.

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Tytuł:: Microbial based assay for specific detection of β-lactam group of antibiotics in milk.
Autorzy:: Das S; Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001 Haryana India.
Kumar N; Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001 Haryana India.
Vishweswaraiah RH; Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001 Haryana India.
Haldar L; The ICFAI University, Tripura, Kamalghat Sadar, Agarthala, 799210 West Tripura, India.
Gaare M; Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001 Haryana India.
Singh VK; Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001 Haryana India.
Puniya AK; Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001 Haryana India.
Źródło:: Journal of food science and technology [J Food Sci Technol] 2014 Jun; Vol. 51 (6), pp. 1161-6. Date of Electronic Publication: 2011 Dec 24.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: 2010- : New Delhi : Springer (India)
Original Publication: Mysore : Association Of Food Scientists And Technologists
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Contributed Indexing:: Keywords: Assay; Bacillus; Induction; Spore; β-lactam; β-lactamase
Entry Date(s):: Date Created: 20140531 Date Completed: 20140530 Latest Revision: 20211021
Update Code:: 20221216
PubMed Central ID:: PMC4033746
DOI:: 10.1007/s13197-011-0609-4
PMID:: 24876650
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The spore forming Bacillus cereus (66) was screened for the induction of β-lactamase in presence of an inducer using iodometric assay. A significant induction in marker enzyme was observed in B. cereus 66 at maximum residual limit (MRL) of penicillin, ampicillin, cloxacillin, amoxicillin, cefalexin, and cephazolin belonging to β-lactam group of antibiotics. A microbial based assay, where enzyme induction was optimized at pH 7.0, temperature 30°C, and whey powder (0.25%) after 4 h of incubation. The spore based assay was tested with milk samples spiked with 6 different β-lactam antibiotics. The results were 100 and 83.33% in correlation with microbial receptor and inhibition based assay, respectively. Overall, spore based assay can be a useful and cost effective tool for the specific detection of β-lactam group of antibiotics in milk.

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