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Tytuł:
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TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.
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Autorzy:
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Redondo AC; Neonatal Intensive Care Unit, Instituto da Criança, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Ceccon ME; Neonatal Intensive Care Unit, Instituto da Criança, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brazil. Electronic address: .
Silveira-Lessa AL; Department of Parasitology, Instituto de Ciências Biomédicas, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Quinello C; Laboratory of Medical Investigation (LIM-36), Instituto da Criança, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Palmeira P; Laboratory of Medical Investigation (LIM-36), Instituto da Criança, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Carvalho WB; Department of Neonatology, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Carneiro-Sampaio M; Department of Pediatrics, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
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Źródło:
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Jornal de pediatria [J Pediatr (Rio J)] 2014 Sep-Oct; Vol. 90 (5), pp. 472-8. Date of Electronic Publication: 2014 May 27.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: Rio de Janeiro, Brazil : Elsevier Editora Ltda
Original Publication: Rio de Janeiro.
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MeSH Terms:
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Cytokines/*blood
Sepsis/*immunology
Toll-Like Receptor 2/*metabolism
Toll-Like Receptor 4/*metabolism
Female ; Flow Cytometry ; Gene Expression ; Humans ; Immunophenotyping ; Infant, Newborn ; Male ; Monocytes/immunology ; Prospective Studies ; Term Birth ; Toll-Like Receptor 2/genetics ; Toll-Like Receptor 4/genetics
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Contributed Indexing:
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Keywords: Imunidade inata; Innate immunity; Monocytes; Monócitos; Newborn; Receptor 2 toll-like; Receptor 4 toll-like; Recém-nascidos; Sepse/imunologia; Sepsis/immunology; Toll-like receptor 2; Toll-like receptor 4
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Substance Nomenclature:
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0 (Cytokines)
0 (Toll-Like Receptor 2)
0 (Toll-Like Receptor 4)
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Entry Date(s):
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Date Created: 20140601 Date Completed: 20150302 Latest Revision: 20200413
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Update Code:
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20240104
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DOI:
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10.1016/j.jped.2013.12.012
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PMID:
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24878008
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Objective: To analyze toll-like receptor (TLR)-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.
Methods: This prospective study included 27 full-term newborns aged 8 to 29 days, with clinical and laboratory diagnosis of late-onset sepsis. Ten newborns (37%) had positive cultures. Cytokines were measured by cytometric bead array in peripheral blood, while TLR-2, TLR-4 expression, and median fluorescence intensity (MFI) were determined by immunophenotyping peripheral whole blood monocytes, and were analyzed with a BD FACSDiva flow cytometer (Becton, Dickinson and Company, USA). A comparison was performed with healthy adults.
Results: Microorganisms were identified in 37% of these septic newborns, and all of them had high levels of pro-inflammatory cytokines (IL-8, IL-6, IL-1β) and anti-inflammatory cytokine (IL-10) corroborating the inflammatory/septic process. In monocytes, the frequency of TLR-4 expression was higher in infected newborns (p = 0.01).
Conclusion: This study investigated the innate immune response in septic newborns. Septic newborns that relied almost exclusively on the innate immune system showed little in vivo response at monocyte activation, suggesting impaired immune response and increased susceptibility to infection.
(Copyright © 2014 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.)
