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Tytuł:
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Clinical significance of molecular methods in the diagnosis of imported malaria in returning travelers in Serbia.
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Autorzy:
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Dakić Z; Department of Microbiology, Clinical Center of Serbia, Belgrade, Serbia.
Ivović V; Center for Parasitic Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.
Pavlović M; Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Clinic for Infectious and Tropical Diseases, Clinical Center of Serbia, Belgrade, Serbia.
Lavadinović L; Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Clinic for Infectious and Tropical Diseases, Clinical Center of Serbia, Belgrade, Serbia.
Marković M; Center for Parasitic Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.
Djurković-Djaković O; Center for Parasitic Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia. Electronic address: .
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Źródło:
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International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2014 Dec; Vol. 29, pp. 24-30. Date of Electronic Publication: 2014 Oct 12.
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Typ publikacji:
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Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: Hamilton, ON : Elsevier
Original Publication: Hamilton, ON : Decker, c1996-
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MeSH Terms:
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Real-Time Polymerase Chain Reaction*
Travel*
Malaria/*diagnosis
Adult ; Aged ; Female ; Humans ; Malaria, Falciparum/diagnosis ; Male ; Microscopy ; Middle Aged ; Parasitemia/diagnosis ; Plasmodium/genetics ; Plasmodium/isolation & purification ; Sensitivity and Specificity ; Serbia ; Species Specificity ; Young Adult
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Contributed Indexing:
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Keywords: Diagnosis; Malaria; Microscopy; Parasitemia; Plasmodium species; Quantitative real-time PCR
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Entry Date(s):
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Date Created: 20141015 Date Completed: 20160115 Latest Revision: 20191210
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Update Code:
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20240104
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DOI:
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10.1016/j.ijid.2014.08.013
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PMID:
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25312981
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Objectives: The goal of this study was to assess the clinical significance of conventional and PCR-based molecular diagnosis in patients with imported malaria in Serbia.
Methods: Giemsa microscopy, the rapid diagnostic test, and quantitative real-time PCR (qPCR) were used to detect Plasmodium species in 109 whole-blood samples from patients after their return from malaria endemic areas, including those clinically suspected for malaria (n=97) and healthy travelers (n=12) examined as part of epidemiological surveillance.
Results: A total of 45 patients were diagnosed with malaria: 42 (93.3%) by microscopy and three (6.7%) additional ones by qPCR. The agreement between the results of species-specific qPCR and microscopy was 73.3%; it was as high as 90.6% for Plasmodium falciparum infections. Follow-up analysis demonstrated persistence of Plasmodium sp DNA for a mean 6 days after the disappearance of parasitemia on microscopy.
Conclusions: Due to its sensitivity and specificity, qPCR is a helpful method complementary to microscopy, particularly in cases of low parasitemia. In addition, it is superior to microscopy for species identification.
(Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.)
