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Tytuł:
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Visinin-like peptide 1 in adrenal gland of the rat. Gene expression and its hormonal control.
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Autorzy:
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Trejter M; Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
Hochol A; Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
Tyczewska M; Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
Ziolkowska A; Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
Jopek K; Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
Szyszka M; Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
Malendowicz LK; Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland. Electronic address: .
Rucinski M; Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
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Źródło:
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Peptides [Peptides] 2015 Jan; Vol. 63, pp. 22-9. Date of Electronic Publication: 2014 Nov 06.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: New York, NY : Elsevier Science Inc.
Original Publication: Fayetteville, N. Y., Ankho International.
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MeSH Terms:
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Gene Expression*
Adrenal Glands/*metabolism
Neurocalcin/*metabolism
Adrenocorticotropic Hormone/pharmacology ; Adrenocorticotropic Hormone/physiology ; Animals ; Dexamethasone/pharmacology ; Female ; Glucocorticoids/pharmacology ; Male ; Neurocalcin/genetics ; Rats, Wistar
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Contributed Indexing:
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Keywords: Adrenal; Adrenal regeneration; Gene expression; Immunocytochemistry; Microarray; Visinin-like peptide 1
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Substance Nomenclature:
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0 (Glucocorticoids)
0 (Neurocalcin)
0 (Vsnl1 protein, rat)
7S5I7G3JQL (Dexamethasone)
9002-60-2 (Adrenocorticotropic Hormone)
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Entry Date(s):
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Date Created: 20141203 Date Completed: 20160905 Latest Revision: 20150404
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Update Code:
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20240104
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DOI:
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10.1016/j.peptides.2014.10.017
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PMID:
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25451331
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VSNL1 encodes the calcium-sensor protein visinin-like 1 and was identified previously as an upregulated gene in a sample set of aldosterone-producing adenomas. Recently, by means of microarray studies we demonstrated high expression of Vsnl1 gene in rat adrenal zona glomerulosa (ZG). Only scanty data are available on the role of this gene in adrenal function as well as on regulation of its expression by factors affecting adrenal cortex structure and function. Therefore we performed relevant studies aimed at clarifying some of the above issues. By Affymetrix(®) Rat Gene 1.1 ST Array Strip, QPCR and immunohistochemistry we demonstrated that expression levels of Vsnl1 in the rat adrenal ZG are notably higher than in the fasciculata/reticularis zone. In QPCR assay this difference was approximately 10 times higher. Expression of this gene in the rat adrenal gland or adrenocortical cells was acutely down regulated by ACTH, while chronic administration of corticotrophin or dexamethasone did not change Vsnl1 mRNA levels. In enucleation-induced adrenocortical regeneration expression levels of both Vsnl1 and Cyp11b2 were notably lowered and positively correlated. Despite these findings, the physiological significance of adrenal Vsnl1 remains unclear, and requires further investigation.
(Copyright © 2014 Elsevier Inc. All rights reserved.)
