[A role for calreticulin in functioning of mitochondrial ATP-dependent potassium channel].

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Abstrakt

Tytuł:: [A role for calreticulin in functioning of mitochondrial ATP-dependent potassium channel].
Autorzy:: Shigaeva MI
Talanov EIu
Venediktova NI
Murzaeva SV
Mironova GD
Źródło:: Biofizika [Biofizika] 2014 Sep-Oct; Vol. 59 (5), pp. 887-94.
Typ publikacji:: English Abstract; Journal Article; Research Support, Non-U.S. Gov't
Język:: Russian
Imprint Name(s):: Original Publication: Moskva : Izdatelstvo Nauka
MeSH Terms:: Adenosine Triphosphatases/*metabolism
Calbindin 2/*metabolism
Cation Transport Proteins/*metabolism
Mitochondria, Liver/*metabolism
Mitochondrial Membranes/*metabolism
Mitochondrial Proteins/*metabolism
Animals ; Antibodies/pharmacology ; Calbindin 2/antagonists & inhibitors ; Male ; Mitochondrial Proteins/antagonists & inhibitors ; Rats ; Rats, Wistar ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substance Nomenclature:: 0 (Antibodies)
0 (Calb2 protein, rat)
0 (Calbindin 2)
0 (Cation Transport Proteins)
0 (Mitochondrial Proteins)
EC 3.6.1.- (Adenosine Triphosphatases)
EC 3.6.1.- (potassium transporting ATPase)
Entry Date(s):: Date Created: 20150304 Date Completed: 20150331 Latest Revision: 20150303
Update Code:: 20240104
PMID:: 25730969
: Czasopismo naukowe

Homology of the amino acid sequence of the mitochondrial potassium-transporting protein (MW 57kDa), having the properties of a channel subunit of the mitochondrial ATP-dependent potassium channel, and calreticulin (MW 55kDa) was detected by MALDI-TOF-TOF analysis method. Inhibitory analysis of ATP-dependent potassium transport in mitochondria with polyclonal antibodies to calreticulin was carried out. A dose-dependent inhibition of potassium transport in mitochondria by these antibodies was shown. The maximum value of inhibition was 55-60%. Based on these data it is hypothesized that at least two types of ATP-sensitive potassium channels are localized in mitochdndrial membrane. It is expected that the type of mitochondrial ATP-dependent potassium channel, which includes homologous calreticulin protein is localized mainly at mitochondrial and reticulum membrane contact sites.

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