Nuclear pores. Architecture of the nuclear pore complex coat.

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Abstrakt

Tytuł:: Nuclear pores. Architecture of the nuclear pore complex coat.
Autorzy:: Stuwe T; Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA. These authors contributed equally to this work.
Correia AR; Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA. These authors contributed equally to this work.
Lin DH; Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.
Paduch M; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.
Lu VT; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.
Kossiakoff AA; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.
Hoelz A; Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA. .
Źródło:: Science (New York, N.Y.) [Science] 2015 Mar 06; Vol. 347 (6226), pp. 1148-52. Date of Electronic Publication: 2015 Feb 12.
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Język:: English
Imprint Name(s):: Publication: : Washington, DC : American Association for the Advancement of Science
Original Publication: New York, N.Y. : [s.n.] 1880-
MeSH Terms:: Nuclear Pore/*ultrastructure
Nuclear Pore Complex Proteins/*chemistry
Saccharomyces cerevisiae/*ultrastructure
Saccharomyces cerevisiae Proteins/*chemistry
Crystallography, X-Ray ; Humans ; Protein Structure, Secondary ; Protein Structure, Tertiary
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Grant Information:: 5 T32 GM07616 United States GM NIGMS NIH HHS; AGM-12006 United States PHS HHS; R01 GM117360 United States GM NIGMS NIH HHS; ACB-12002 United States PHS HHS; U54 GM087519 United States GM NIGMS NIH HHS; R01 GM111461 United States GM NIGMS NIH HHS; U01 GM094588 United States GM NIGMS NIH HHS
Molecular Sequence:: PDB 4XMM; 4XMN
Substance Nomenclature:: 0 (Nuclear Pore Complex Proteins)
0 (Saccharomyces cerevisiae Proteins)
Entry Date(s):: Date Created: 20150307 Date Completed: 20150406 Latest Revision: 20190918
Update Code:: 20240104
PubMed Central ID:: PMC5180592
DOI:: 10.1126/science.aaa4136
PMID:: 25745173
: Czasopismo naukowe

Pełny tekst

The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. Despite half a century of structural characterization, the architecture of the NPC remains unknown. Here we present the crystal structure of a reconstituted ~400-kilodalton coat nucleoporin complex (CNC) from Saccharomyces cerevisiae at a 7.4 angstrom resolution. The crystal structure revealed a curved Y-shaped architecture and the molecular details of the coat nucleoporin interactions forming the central "triskelion" of the Y. A structural comparison of the yeast CNC with an electron microscopy reconstruction of its human counterpart suggested the evolutionary conservation of the elucidated architecture. Moreover, 32 copies of the CNC crystal structure docked readily into a cryoelectron tomographic reconstruction of the fully assembled human NPC, thereby accounting for ~16 megadalton of its mass.
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