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Tytuł:
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Adipose Triglyceride Lipase, Not Hormone-Sensitive Lipase, Is the Primary Lipolytic Enzyme in Fasting Elephant Seals (Mirounga angustirostris).
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Autorzy:
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Fowler MA; Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, California; 2Department of Biology, Sonoma State University, Rohnert Park, California; 3Division of Endocrinology, Gerontology, and Metabolism, Stanford University and Veterans Affairs Palo Alto Health Care System, Palo Alto, California.
Costa DP
Crocker DE
Shen WJ
Kraemer FB
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Źródło:
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Physiological and biochemical zoology : PBZ [Physiol Biochem Zool] 2015 May-Jun; Vol. 88 (3), pp. 284-94. Date of Electronic Publication: 2015 Jan 28.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
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Język:
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English
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Imprint Name(s):
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Original Publication: Chicago, IL : University of Chicago Press, c1999-
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MeSH Terms:
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Adipose Tissue/*enzymology
Lipase/*metabolism
Seals, Earless/*metabolism
Sterol Esterase/*metabolism
Animals ; Fasting ; Fatty Acids, Nonesterified/metabolism ; Female ; Lactation/metabolism ; Milk/chemistry ; Molting
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Grant Information:
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I01 BX000398 United States BX BLRD VA
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Substance Nomenclature:
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0 (Fatty Acids, Nonesterified)
EC 3.1.1.13 (Sterol Esterase)
EC 3.1.1.3 (Lipase)
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Entry Date(s):
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Date Created: 20150411 Date Completed: 20160405 Latest Revision: 20161025
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Update Code:
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20240104
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DOI:
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10.1086/680079
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PMID:
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25860827
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Little is known about the mechanisms that allow capital breeders to rapidly mobilize large amounts of body reserves. Northern elephant seals (Mirounga angustirostris) utilize fat reserves for maternal metabolism and to create high fat milk for the pup. Hormone-sensitive lipase (HSL) has been hypothesized to be an important lipolytic enzyme in fasting seals, but the activity of HSL and adipose triglyceride lipase (ATGL) has not been quantified in fasting adult seals, nor has their relationship to milk lipid content been assessed. Blubber and milk samples were obtained from 18 early lactation and 19 late lactation females, as well as blubber from five early and five late molting female seals. Blubber lipolytic activity was assessed with radiometric assays. HSL activity was negligible in seal blubber at all fasting stages. Total triglyceride lipase activity was stable among early and late lactation and early molt but increased in late molting seals. Relative abundance of ATGL protein increased across fasting, but neither activity nor relative protein levels were related to circulating nonesterified fatty acids or milk lipid content, suggesting the possibility of other regulatory pathways between lipolytic activity and milk lipid content. These results demonstrate that HSL is not the primary lipolytic enzyme in fasting adult female seals and that ATGL contributes more to lipolysis than HSL.
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