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Tytuł pozycji:

Fluorescent Visualisation of Oxytocin in the Hypothalamo-neurohypophysial/-spinal Pathways After Chronic Inflammation in Oxytocin-Monomeric Red Fluorescent Protein 1 Transgenic Rats.

Tytuł :
Fluorescent Visualisation of Oxytocin in the Hypothalamo-neurohypophysial/-spinal Pathways After Chronic Inflammation in Oxytocin-Monomeric Red Fluorescent Protein 1 Transgenic Rats.
Autorzy :
Matsuura T; Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Kawasaki M; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Hashimoto H; Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Ishikura T; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Yoshimura M; Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Ohkubo JI; Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Maruyama T; Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Motojima Y; Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Sabanai K; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Mori T; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Ohnishi H; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Sakai A; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Ueta Y; Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
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Źródło :
Journal of neuroendocrinology [J Neuroendocrinol] 2015 Jul; Vol. 27 (7), pp. 636-46.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Publication: <2010->: Malden, MA : Wiley & Sons
Original Publication: Eynsham, Oxon, UK : Oxford University Press, c1989-
MeSH Terms :
Arthritis/*metabolism
Inflammation/*metabolism
Oxytocin/*metabolism
Paraventricular Hypothalamic Nucleus/*metabolism
Signal Transduction/*physiology
Spinal Cord/*metabolism
Supraoptic Nucleus/*metabolism
Animals ; Chronic Disease ; Disease Models, Animal ; Luminescent Agents ; Luminescent Proteins/genetics ; Male ; Oxytocin/genetics ; Rats ; Rats, Transgenic ; Rats, Wistar
Contributed Indexing :
Keywords: arthritic rats; chronic inflammation; fluorescent protein; oxytocin
Substance Nomenclature :
0 (Luminescent Agents)
0 (Luminescent Proteins)
0 (red fluorescent protein)
50-56-6 (Oxytocin)
Entry Date(s) :
Date Created: 20150507 Date Completed: 20160329 Latest Revision: 20150623
Update Code :
20210210
DOI :
10.1111/jne.12290
PMID :
25943916
Czasopismo naukowe
Oxytocin (OXT) is a well-known neurohypophysial hormone that is synthesised in the paraventricular (PVN) and supraoptic nuclei (SON) of the hypothalamus. The projection of magnocellular neurosecretory cells, which synthesise OXT and arginine vasopressin in the PVN and SON, to the posterior pituitary plays an essential role in mammalian labour and lactation through its peripheral action. However, previous studies have shown that parvocellular OXTergic cells in the PVN, which project to the medulla and spinal cord, are involved in various physiological functions (e.g. sensory modulation and autonomic). In the present study, we examined OXT expression in the PVN, SON and spinal cord after chronic inflammation from adjuvant arthritis (AA). We used transgenic rats that express OXT and the monomeric red fluorescent protein 1 (mRFP1) fusion gene to visualise both the magnocellular and parvocellular OXTergic pathways. OXT-mRFP1 fluorescence intensity was significantly increased in the PVN, SON, dorsal horn of the spinal cord and posterior pituitary in AA rats. The levels of OXT-mRFP1 mRNA were significantly increased in the PVN and SON of AA rats. These results suggested that OXT was up-regulated in both hypothalamic magnocellular neurosecretory cells and parvocellular cells by chronic inflammation, and also that OXT in the PVN-spinal pathway may be involved in sensory modulation. OXT-mRFP1 transgenic rats are a very useful model for visualising the OXTergic pathways from vesicles in a single cell to terminals in in vitro preparations.
(© 2015 British Society for Neuroendocrinology.)
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