-
Tytuł:
-
Genetic and clinical characterization of canine leishmaniasis caused by Leishmania (Leishmania) infantum in northeastern Argentina.
-
Autorzy:
-
Barroso PA; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta (UNSa) / Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bolivia 5150, A4408FVY Salta, Argentina; Instituto de Investigaciones en Enfermedades Tropicales, Sede Regional Orán, UNSa, Alvarado 751, A4530ANQ San Ramón de la Nueva Orán, Argentina. Electronic address: .
Nevot MC; Veterinaria del Oeste, Lavalle 257, N3300ONN Posadas, Argentina. Electronic address: .
Hoyos CL; Instituto de Investigaciones en Enfermedades Tropicales, Sede Regional Orán, UNSa, Alvarado 751, A4530ANQ San Ramón de la Nueva Orán, Argentina. Electronic address: .
Locatelli FM; Department of Parasitology, Kochi Medical School, Kochi University, Kohasu, Oko, Nankoku, 783-8505 Kochi, Japan. Electronic address: .
Lauthier JJ; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta (UNSa) / Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bolivia 5150, A4408FVY Salta, Argentina. Electronic address: .
Ruybal P; Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires / CONICET, Paraguay 2155, C1121ABG Buenos Aires, Argentina. Electronic address: .
Cardozo RM; Coordinación de Gestión Epidemiológica, Ministerio de Salud Pública, Centro Cívico Grand Bourg, 4400 Salta, Argentina. Electronic address: cardozo_.
Russo PD; Veterinaria del Oeste, Lavalle 257, N3300ONN Posadas, Argentina. Electronic address: .
Vassiliades CN; Veterinaria del Oeste, Lavalle 257, N3300ONN Posadas, Argentina. Electronic address: .
Mora MC; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta (UNSa) / Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bolivia 5150, A4408FVY Salta, Argentina. Electronic address: .
Estévez JO; Veterinaria del Oeste, Lavalle 257, N3300ONN Posadas, Argentina. Electronic address: .
Hashiguchi Y; Department of Parasitology, Kochi Medical School, Kochi University, Kohasu, Oko, Nankoku, 783-8505 Kochi, Japan; Centro de Biomedicina, Universidad Central del Ecuador y Proyecto Prometeo, SNESCYT, Sodiro N14-121 e Iquique, Quito, Ecuador. Electronic address: .
Korenaga M; Department of Parasitology, Kochi Medical School, Kochi University, Kohasu, Oko, Nankoku, 783-8505 Kochi, Japan. Electronic address: .
Basombrío MA; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta (UNSa) / Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bolivia 5150, A4408FVY Salta, Argentina. Electronic address: .
Marco JD; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta (UNSa) / Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bolivia 5150, A4408FVY Salta, Argentina; Cátedra de Microbiología, Facultad de Ciencias Exactas, UNSa, Bolivia 5150, A4408FVY Salta, Argentina. Electronic address: .
-
Źródło:
-
Acta tropica [Acta Trop] 2015 Oct; Vol. 150, pp. 218-23. Date of Electronic Publication: 2015 Aug 13.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Amsterdam : Elsevier
Original Publication: Basel.
-
MeSH Terms:
-
Dog Diseases/*epidemiology
Leishmania infantum/*isolation & purification
Leishmaniasis, Visceral/*veterinary
Animals ; Argentina/epidemiology ; Disease Reservoirs ; Dogs ; Female ; Leishmania infantum/genetics ; Leishmaniasis, Visceral/epidemiology ; Male ; Polymerase Chain Reaction ; Zoonoses
-
Contributed Indexing:
-
Keywords: Argentina; Canine; Cytochrome b; Dog; Leishmania infantum; Typing
-
Entry Date(s):
-
Date Created: 20150817 Date Completed: 20160523 Latest Revision: 20181202
-
Update Code:
-
20240104
-
DOI:
-
10.1016/j.actatropica.2015.08.007
-
PMID:
-
26277067
-
Leishmaniases comprise zoonotic diseases caused by protozoan flagellates of the Leishmania genus. They are endemic to South America, and the visceral form has been recently reported in Argentina. Dogs can play different roles in the Leishmania transmission cycles, depending mainly on the species of parasite involved. Here we focused on the clinical characterization of canine leishmaniasis (CanL) in Northeast Argentina and on the molecular typing of its etiological agent. The nested polymerase chain reaction and sequence analysis of the Leishmania cytochrome b (cyt b) gene was performed on DNA templates purified from lymph nodes, bone marrow or spleen aspirates obtained from 48 dogs previously diagnosed by the observation of Leishmania amastigotes on smears from these aspirates. Their clinical and epidemiological data were also recorded. Systemic abnormalities were observed in 46 subjects (95.8%), most frequently lymphadenopathy, and emaciation (89.6 and 75%). Furthermore, 87% also presented tegumentary abnormalities, such as alopecia (54.2%) or secondary skin lesions (47.9%), among others. Twenty three dogs were positive for cyt b amplification. The sequence analysis showed the presence of two genotypes, LiA1 and LiA2, assigned to Leishmania (Leishmania) infantum, with 99.9 and 100% homology with the reference strain MHOM/TN/80/IPT1 respectively. LiA1 was identified in 18 cases (78.3%) and LiA2 in five (21.7%). Two cyt b variants of L. (L.) infantum were incriminated as the causative agents of CanL cases from three cities: Posadas, Garupá, and Ituzaingó. All three cities are located in the northeastern area of the country, where these parasites seem to be spreading in urban areas.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
