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Tytuł:
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Differential Regulation of Specific Sphingolipids in Colon Cancer Cells during Staurosporine-Induced Apoptosis.
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Autorzy:
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del Solar V; Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Lizardo DY; Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Li N; Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Hurst JJ; Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Brais CJ; Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Atilla-Gokcumen GE; Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA. Electronic address: .
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Źródło:
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Chemistry & biology [Chem Biol] 2015 Dec 17; Vol. 22 (12), pp. 1662-70.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: 2001- : Maryland Heights, MO : Elsevier
Original Publication: London ; Philadelphia, PA : Current Biology Ltd., c1994-
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MeSH Terms:
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Apoptosis/*drug effects
Apoptosis/*physiology
Sphingolipids/*metabolism
Staurosporine/*pharmacology
Cell Survival ; Colonic Neoplasms/drug therapy ; HCT116 Cells ; Humans ; Mass Spectrometry ; Molecular Structure ; Polymerase Chain Reaction ; Sphingolipids/analysis
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Substance Nomenclature:
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0 (Sphingolipids)
H88EPA0A3N (Staurosporine)
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Entry Date(s):
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Date Created: 20151222 Date Completed: 20160726 Latest Revision: 20151221
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Update Code:
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20240104
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DOI:
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10.1016/j.chembiol.2015.11.004
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PMID:
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26687483
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Apoptosis is accompanied by distinct morphological changes at the plasma and organelle membrane level. Involvement of certain lipids in apoptosis has been established; however, we have limited understanding of the specific lipid structures that participate in this process. We used untargeted comparative lipidomics to study the changes in lipid composition during staurosporine-induced apoptosis in HCT-116. Our results revealed that ceramides, dihydroceramides, and sphingomyelins, with defined acyl chains, constitute the majority of changes in the lipidome. Expression levels and activities of enzymes responsible for the biosynthesis of lipids that change suggest that de novo synthesis causes these specific changes. Further analysis of the lipidome during apoptosis in other cancer and non-cancer cell lines suggested that accumulation of ceramides and dihydroceramides is specific to cancer cells. Taken together, our data propose that these molecules are regulated at the lipid-specific level during apoptosis and that this regulation differs between cancer and non-cancer cells.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
